Human STING/TMEM173 PE-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human STING/TMEM173 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 723505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human STING/TMEM173
    Ala215-Ser379
    Accession # Q86WV6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 PE‑conjugated Monoclonal Antibody (Catalog # IC7169P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in THP‑1 Human Cell Line by Flow Cytometry. THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 PE‑conjugated Monoclonal Antibody (Catalog # IC7169P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 PE‑conjugated Monoclonal Antibody (Catalog # IC7169P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STING/TMEM173
STING (Stimulator of Interferon Genes), also called ERIS, MPYS, or MITA and designated TMEM173, is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found predominantly in the endoplasmic reticulum. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human and mouse STING share 76% aa sequence identity.
  • Long Name:
    Stimulator of Interferon Genes Protein/Transmembrane protein 173
  • Entrez Gene IDs:
    340061 (Human); 72512 (Mouse)
  • Alternate Names:
    endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; NET23; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; STING; STINGhSTING; TMEM173; transmembrane protein 173
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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