Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 723505
View all STING/TMEM173 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STING/TMEM173
Ala215-Ser379
Accession # Q86WV6

Specificity

Detects human STING/TMEM173 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human STING/TMEM173 Antibody

Detection of Human STING/TMEM173 antibody by Western Blot.

Detection of Human STING/TMEM173 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of STING/TMEM173 antibody in Human PBMC Monocytes antibody by Flow Cytometry.

Detection of STING/TMEM173 in Human PBMC Monocytes by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of STING/TMEM173 antibody in THP-1 Human Cell Line antibody by Flow Cytometry.

Detection of STING/TMEM173 in THP‑1 Human Cell Line by Flow Cytometry.

THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Detection of STING/TMEM173 antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of STING/TMEM173 in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

STING/TMEM173 antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

STING/TMEM173 in U937 Human Cell Line.

STING/TMEM173 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse STING/TMEM173 by Western Blot

Detection of Mouse STING/TMEM173 by Western Blot

CYLD interacts with STING in a ubiquitination-dependent manner.(A) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody and then immunoblotted with the indicated antibodies. (B) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, cell lysates were immunoprecipitated with an anti-HA antibody and then immunoblotted with the indicated antibodies. (C and D) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody (C) or an anti-HA antibody (D) and then immunoblotted with the indicated antibodies. (E) HEK293T cells were transfected with the indicated plasmids. Thirty-six hours after transfection, the cell lysates were immunoprecipitated with an anti-Myc antibody and then immunoblotted with the indicated antibodies. (F) After stimulation with HSV-1 (MOI = 10) for the indicated time periods in the presence of MG132 (10 μM), lysates of MEFs were immunoprecipitated with an anti-STING antibody and then immunoblotted with the indicated antibodies. (G) HeLa cells were transfected with empty vector or Flag-tagged RNF5 for 24 h and then stained with the indicated antibodies before imaging by confocal microscopy. STING (Green) (R&D Systems); CYLD (Red) (Abcam); Nucleus (Blue). Scale bars represent 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30388174), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human STING/TMEM173 by Western Blot

Detection of Human STING/TMEM173 by Western Blot

HCT116 cells surviving PARP1 depletion activate innate immune signaling.(A-B) RNA-Seq data from HCT116EV and HCT116PARP1-/- cells (clones C2 and C4) was analyzed using Gene Set Enrichment Analysis (GSEA). The top category of differentially expressed genes was “Interferon Alpha Response”. The enrichment plot is shown in (A) and the heat map for the 97 mRNAs in this category in shown in (B). Map was generated using Cufflinks software (version 2.2.1) and shows absolute expression values independently normalized and analyzed for each comparison pair (EV/C2 and EV/C4). (C) The same RNA-Seq dataset was analyzed using Ingenuity Pathway Analysis (IPA). A representative plot highlighting enrichment for Interferon-Stimulated Genes (ISGs) is shown. (D) The induction of multiple ISGs observed by RNA-Seq was confirmed by q-RT-PCR. Bars represent the average and standard deviation of quadruplicate samples in each experiment and data is representative of 2–4 independent experiments. (E-F) The induction of factors involved in the sensing/signaling of cytoplasmic nucleic acids observed by RNA-Seq was confirmed by Q-RT-PCR (E). Bars represent the average and standard deviation of quadruplicate samples in each experiment and data is representative of 2–3 independent experiments. Protein expression for the same factors was assessed by immunoblotting (F). Blots are representative of 2–3 independent experiments. (G) Fixed cells were stained with an antibody to IRF3 and counterstained with DAPI. Images are representative of 5 random fields per slide. The experiment was repeated twice with similar results. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29590171), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human STING/TMEM173 by Western Blot

Detection of Human STING/TMEM173 by Western Blot

ISG induction in cells surviving PARP1 depletion is dependent on RIG-I and MAVS.(A-E) To assess the efficiency of RNA silencing to STING (A), RIG-I (B), MDA-5 (C), TLR3 (D) or MAVS (E), cells transfected with the specific pooled siRNAs and control cells transfected with a scrambled siRNA or untreated were harvested 4 days after transfection and probed with the indicated antibodies. GAPDH (A, B, C, E) or alpha -tubulin (D) served as loading controls. In blots where multiple bands are observed (A, D, E), black arrows point to specific bands and asterisks to unspecific bands. (F) The expression of ISGs OAS1 and IFIT3 was quantified by qRT-PCR after knock down. Data is normalized to the expression in HCT116EV cells (= 1). Bars represent the average and standard deviation of quadruplicates. Data is representative of two independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29590171), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of STING/TMEM173 by Immunoprecipitation.

PMA-treated THP-1 lysates were prepared, and immunoprecipitation was performed using 2.0 μg of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) pre-coupled to Dynabeads protein G. Immunoprecipitated STING/TMEM173 was detected with a Rabbit Anti-STING/TMEM173 antibody. For western blot, the Rabbit Anti-STING/TMEM173 antibody was used at 1/1000. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. *=monoclonal antibody, **=recombinant antibody. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for Human STING/TMEM173 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Immunoprecipitation

2 µg/mL
Sample: Cell lysate of PMA-treated THP-1 human acute monocytic leukemia cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) monocytes, THP‑1 human acute monocytic leukemia cell line, and U937 human histiocytic lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.2 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line

Reviewed Applications

Read 1 review rated 3 using MAB7169 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STING/TMEM173

STING (Stimulator of Interferon Genes), also called ERIS, MPYS, or MITA and designated TMEM173, is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found predominantly in the endoplasmic reticulum. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human and mouse STING share 76% aa sequence identity.

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173

Entrez Gene IDs

340061 (Human); 72512 (Mouse)

Gene Symbol

STING1

UniProt

Q86WV6 (Human)

Additional STING/TMEM173 Products

Product Documents for Human STING/TMEM173 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human STING/TMEM173 Antibody

For research use only

Citations for Human STING/TMEM173 Antibody

Customer Reviews for Human STING/TMEM173 Antibody (1)

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  • Human STING/TMEM173 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: U2OS cells and HeLa cells
    Species: Human
    Verified Customer | Posted 10/11/2020
    ALT cells, U2OS shown here, are shown to lack STING. Based on this the apparent band of interest of 40-42kD seems to be migrating further up closer to the 50kD instead of the 37kD ladder.
    Antibody used at 0.105ug/mL or 1/2000. Blocked and incubated with 5% milk in TBST overnight at 4C. Blot detected using PICO Plus chemiluminescence reagent (Life Technologies).
    Human STING/TMEM173 Antibody MAB7169

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