Human STING/TMEM173 Antibody
Human STING/TMEM173 Antibody Summary
Ala215-Ser379
Accession # Q86WV6
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human STING/TMEM173 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of STING/TMEM173 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Detection of STING/TMEM173 in THP‑1 Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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Detection of STING/TMEM173 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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STING/TMEM173 in U937 Human Cell Line. STING/TMEM173 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STING/TMEM173
STING (Stimulator of Interferon Genes), also called ERIS, MPYS, or MITA and designated TMEM173, is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found predominantly in the endoplasmic reticulum. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human and mouse STING share 76% aa sequence identity.
Product Datasheets
Citations for Human STING/TMEM173 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
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YAP inhibits HCMV replication by impairing STING-mediated nuclear transport of the viral genome
Authors: JH Lee, M Kwon, WY Lim, CR Yoo, Y Yoon, D Han, JH Ahn, K Yoon
PloS Pathogens, 2022;18(12):e1011007.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
UXT attenuates the CGAS-STING1 signaling by targeting STING1 for autophagic degradation
Authors: M Pan, Y Yin, T Hu, X Wang, T Jia, J Sun, Q Wang, W Meng, J Zhu, C Dai, H Hu, C Wang
Autophagy, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Tousled-Like Kinases Suppress Innate Immune Signaling Triggered by Alternative Lengthening of Telomeres
Authors: S Segura-Bay, M Villamor-P, CS Attolini, LM Koenig, M Sanchiz-Ca, SJ Boulton, TH Stracker
Cell Rep, 2020;32(5):107983.
Species: Human
Sample Types: Cell Lysates
Applications: ChIP -
The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus
Authors: M Gentili, X Lahaye, F Nadalin, GFP Nader, E Puig Lomba, S Herve, NS De Silva, DC Rookhuizen, E Zueva, C Goudot, M Maurin, A Bochnakian, S Amigorena, M Piel, D Fachinetti, A Londoño-Va, N Manel
Cell Rep, 2019;26(9):2377-2393.e13.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
PARP1 depletion induces RIG-I-dependent signaling in human cancer cells
Authors: R Ghosh, S Roy, S Franco
PLoS ONE, 2018;13(3):e0194611.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HPV18 Persistence Impairs Basal and DNA Ligand-Mediated IFN-? and IFN-?Production through Transcriptional Repression of Multiple Downstream Effectors of Pattern Recognition Receptor Signaling
Authors: S Albertini, I Lo Cigno, F Calati, M De Andrea, C Borgogna, V Dell'Oste, S Landolfo, M Gariglio
J. Immunol., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human cytomegalovirus-encoded US9 targets MAVS and STING signaling to evade type I interferon immune responses
Authors: HJ Choi, A Park, S Kang, E Lee, TA Lee, EA Ra, J Lee, S Lee, B Park
Nat Commun, 2018;9(1):125.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
UBXN3B positively regulates STING-mediated antiviral immune responses
Authors: L Yang, L Wang, H Ketkar, J Ma, G Yang, S Cui, T Geng, DG Mordue, T Fujimoto, G Cheng, F You, R Lin, E Fikrig, P Wang
Nat Commun, 2018;9(1):2329.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway
Authors: L Zhang, N Wei, Y Cui, Z Hong, X Liu, Q Wang, S Li, H Liu, H Yu, Y Cai, Q Wang, J Zhu, W Meng, Z Chen, C Wang
PLoS Pathog., 2018;14(11):e1007435.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Extrachromosomal telomere repeat DNA is linked to ALT development via cGAS-STING DNA sensing pathway
Authors: YA Chen, YL Shen, HY Hsia, YP Tiang, TL Sung, LY Chen
Nat. Struct. Mol. Biol., 2017;0(0):.
Species: Human
Sample Types: Cell Extracts
Applications: Western Blot -
Evasion of the STING DNA sensing pathway by the VP11/12 of herpes simplex virus type 1
Authors: T Deschamps, M Kalamvoki
J. Virol., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents
Authors: J Gaston, L Cheradame, V Yvonnet, O Deas, MF Poupon, JG Judde, S Cairo, V Goffin
Oncotarget, 2016;7(47):77205-77224.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
Authors: Berg R, Rahbek S, Kofod-Olsen E, Holm C, Melchjorsen J, Jensen D, Hansen A, Jorgensen L, Ostergaard L, Tolstrup M, Larsen C, Paludan S, Jakobsen M, Mogensen T
PLoS ONE, 2014;9(1):e84513.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
Authors: Schoggins J, MacDuff D, Imanaka N, Gainey M, Shrestha B, Eitson J, Mar K, Richardson R, Ratushny A, Litvak V, Dabelic R, Manicassamy B, Aitchison J, Aderem A, Elliott R, Garcia-Sastre A, Racaniello V, Snijder E, Yokoyama W, Diamond M, Virgin H, Rice C
Nature, 2014;505(7485):691-5.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation.
Authors: Kalamvoki M, Roizman B
Proc Natl Acad Sci U S A, 2014;111(5):E611-7.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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