Human STING/TMEM173 Antibody MAB7169: R&D Systems

Human STING/TMEM173 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human STING/TMEM173 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 723505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human STING/TMEM173
    Ala215-Ser379
    Accession # Q86WV6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human STING/TMEM173 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STING/TMEM173 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in THP‑1 Human Cell Line by Flow Cytometry.
THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Intracellular Staining by Flow Cytometry
Detection of STING/TMEM173 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunocytochemistry
STING/TMEM173 in U937 Human Cell Line. STING/TMEM173 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Mouse Anti-Human STING/TMEM173 Monoclonal Antibody (Catalog # MAB7169) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STING/TMEM173
STING (Stimulator of Interferon Genes), also called ERIS, MPYS, or MITA and designated TMEM173, is a 40-42 kDa 4-transmembrane protein that mediates both antiviral and MHC-II antigen recognition responses. STING is found predominantly in the endoplasmic reticulum. It acts as an adaptor protein for intracellular viral detection molecules, participating in the induction of type I interferon. It also may play a role in the initiation of apoptosis following MHC-II engagement. Cells known to express STING include B cells, dendritic cells, macrophages, and monocytes. Human STING is 379 amino acids (aa) in length. It contains an N-terminal cytoplasmic region (aa 1-20), four transmembrane segments (aa 21-173), and a C-terminal cytoplasmic domain (aa 174-379). Ubiquitination occurs at Lys150, and phosphorylation occurs at Ser358. STING forms 80 kDa homodimers. There are two potential splice forms, one that shows a 25 aa substitution for aa 1-173, and another that possesses an alternative start site at Met215, coupled to a premature truncation following Arg334. Over aa 215-379, human and mouse STING share 76% aa sequence identity.
  • Long Name:
    Stimulator of Interferon Genes Protein/Transmembrane protein 173
  • Entrez Gene IDs:
    340061 (Human); 72512 (Mouse)
  • Alternate Names:
    endoplasmic reticulum IFN stimulator; Endoplasmic reticulum interferon stimulator; ERIS; FLJ38577; hMITA; Mediator of IRF3 activation; MITA; mitochondrial mediator of IRF3 activation; MPYS; NET23; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; Stimulator of interferon genes protein; STING; STINGhSTING; TMEM173; transmembrane protein 173
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Intracellular STING inactivation sensitizes breast cancer cells to genotoxic agents
    Authors: J Gaston, L Cheradame, V Yvonnet, O Deas, MF Poupon, JG Judde, S Cairo, V Goffin
    Oncotarget, 2016;7(47):77205-77224.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. HSV-1 degrades, stabilizes, requires, or is stung by STING depending on ICP0, the US3 protein kinase, and cell derivation.
    Authors: Kalamvoki M, Roizman B
    Proc Natl Acad Sci U S A, 2014;111(5):E611-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity.
    Authors: Schoggins J, MacDuff D, Imanaka N, Gainey M, Shrestha B, Eitson J, Mar K, Richardson R, Ratushny A, Litvak V, Dabelic R, Manicassamy B, Aitchison J, Aderem A, Elliott R, Garcia-Sastre A, Racaniello V, Snijder E, Yokoyama W, Diamond M, Virgin H, Rice C
    Nature, 2014;505(7485):691-5.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. T cells detect intracellular DNA but fail to induce type I IFN responses: implications for restriction of HIV replication.
    Authors: Berg R, Rahbek S, Kofod-Olsen E, Holm C, Melchjorsen J, Jensen D, Hansen A, Jorgensen L, Ostergaard L, Tolstrup M, Larsen C, Paludan S, Jakobsen M, Mogensen T
    PLoS ONE, 2014;9(1):e84513.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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