Human Syndecan-4 APC-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Syndecan-4 in flow cytometry.
  • Source
    Monoclonal Rat IgG2A Clone # 336304
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
    Ser20-Glu145
    Accession # P31431
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Syndecan‑4 in Activated Lymphocytes in Human PBMCs by Flow Cytometry. Activated lymphocytes in human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human Syndecan‑4 APC‑conjugated Monoclonal Antibody (Catalog # FAB29181A) or (B) Rat IgG2A Allophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Syndecan-4

Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

  • References:
    1. Tkachenko, E. et al. (2005) Circ. Res. 96:488. 
    2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
    3. David, G. et al. (1992) J. Cell Biol. 118:961. 
    4. Choi, S. et al. (2005) J. Biol. Chem. 280:42573. 
    5. Charnaux, N. et al. (2005) FEBS J. 272:1937.  
    6. Schmidt, A. et al., J. Biol. Chem. 280:34441.
    7. Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
    8. Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
    9. Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
    10. Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.
  • Entrez Gene IDs:
    6385 (Human)
  • Alternate Names:
    Amphiglycan; MGC22217; Ryudocan core protein; Ryudocan; SDC4; SYND4ryudocan amphiglycan; syndecan 4 (amphiglycan, ryudocan); syndecan 4; syndecan proteoglycan 4; Syndecan4; Syndecan-4
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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