Human TACE/ADAM17 Ectodomain Antibody

Catalog # Availability Size / Price Qty
AF930
AF930-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Human TACE/ADAM17 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects human TACE/ADAM17 Ectodomain in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) BACE, rhADAM8, rhADAM10, and recombinant mouse ADAM10 is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Insect ovarian cell line T. ni-derived recombinant human TACE/ADAM17
Pro18-Asn671
Accession # P78536
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TACE/ADAM17 Western Blot Standard (Catalog # WBC029)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACE/ADAM17

TACE is a member of the ADAM family that contains A Disintegrin And Metalloprotease-like domain. Like other membrane-anchored ADAMs, TACE consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. In addition to its ability to release the 17 kDa extracellular form of tumor necrosis factor-alpha (TNF-alpha ) from the 26 kDa membrane-anchored TNF-alpha, TACE also plays an essential role in shedding ectodomains from a variety of proteins such as L-Selectin, Transforming Growth Factor-alpha, Amyloid Protein Precursor, and Notch-1 receptor. TACE mRNA is present in virtually every tissue and TACE protein resides both on the cell surface and in the cell.

References
  1. Black, R.A. and J.D. Becherer (1998) in Tumor Necrosis Factor alpha -Converting Enzyme. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 1315.
  2. Primakoff, P. and D.G. Myles (2000) Trends in Genetics 16:83.
Long Name
TNF-alpha Converting Enzyme
Entrez Gene IDs
6868 (Human); 11491 (Mouse)
Alternate Names
ADAM 17; ADAM metallopeptidase domain 17; ADAM metallopeptidase domain 18; ADAM17; ADAM18; CD156b antigen; CD156b; CSVP; disintegrin and metalloproteinase domain-containing protein 17; EC 3.4.24.86; MGC71942; Snake venom-like protease; TACE; TACEcSVP; TNF-alpha convertase; TNF-alpha converting enzyme; TNF-alpha-converting enzyme; tumor necrosis factor, alpha, converting enzyme

Product Datasheets

Citations for Human TACE/ADAM17 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The shedding activity of ADAM17 is sequestered in lipid rafts.
    Authors: Tellier E, Rebsomen L
    Exp. Cell Res., 2006;312(20):3969-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. FHL2 interacts with both ADAM-17 and the cytoskeleton and regulates ADAM-17 localization and activity.
    Authors: Canault M, Tellier E, Bonardo B, Mas E, Aumailley M, Juhan-Vague I, Nalbone G, Peiretti F
    J. Cell. Physiol., 2006;208(2):363-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. ADAMs, a disintegrin and metalloproteinases, mediate shedding of oxytocinase.
    Authors: Ito N, Nomura S, Iwase A, Ito T, Kikkawa F, Tsujimoto M, Ishiura S, Mizutani S
    Biochem. Biophys. Res. Commun., 2004;314(4):1008-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human TACE/ADAM17 Ectodomain Antibody
By Anonymous on 11/17/2018
Application: ICC/IF Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human