Human TCF-2/HNF-1 beta Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3330X Datasheet / COA / SDS

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AF3330X-100UG

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Human TCF-2/HNF-1 beta Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

This antibody detects TCF‑2/HNF‑1 beta in direct ELISAs and Western blots. It is expected to recognize all three TCF‑2 isoforms.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TCFâ€‘2/HNFâ€‘1Î²
Gly51-Gln182
Accession # P35680

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TCF-2/HNF-1 beta

TCF-2, also known as HNF-1 beta (hepatocyte nuclear factor 1 beta ) and VHNF-1, is a member of the HNF-1 homeobox family. By alternative splicing, three isoforms of TCF-2/HNF-1 beta exist. TCF-2/HNF-1 beta binds DNA as a homodimer or heterodimer with TCF-1/HNF-1 alpha. Within the region used as the immunogen, human TCF-2 shares 98% amino acid sequence homology with mouse and rat TCF-2. TCF-2 expression is required for visceral endoderm specification.

Long Name

Transcription Factor 2

Entrez Gene IDs

6928 (Human); 21410 (Mouse); 25640 (Rat)

Alternate Names

FJHN; hepatocyte nuclear factor 1-beta; HNF1 beta A; HNF-1 beta; HNF1 homeobox B; HNF1B; HNF-1B; HNF1beta; HNF-1-beta; HNF2; Homeoprotein LFB3; LFB3; LF-B3; MODY5; MODY5HPC11; TCF2; TCF-2; TCF2transcription factor 2, hepatic; LF-B3; variant hepatic nuclear factor; Transcription factor 2; transcription factor 2, hepatic; Variant hepatic nuclear factor 1; VHNF1