Human TDRKH Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: TDRKH

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins. It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells. TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome. Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for non-coding RNA binding. Human TDRKH is 606 aa in length. It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412). There are four potential splice forms. Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606. Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.

Product Datasheets

Related Research Areas

• Primordial and Mature Germ Cells