Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all TFF3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654

Specificity

Detects human TFF3 in Western blots. In this format, less than 1% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human TFF3 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human TFF3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF3

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease‑resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22 ‑ 80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond‑mediated loops (aa 30 ‑ 72). Human TFF3 exists as both a monomer and disulfide‑linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29 ‑ 80. Over aa 21 ‑ 80, human TFF3 shares 77%, 68% and 72% aa identity with mouse, canine and rat TFF3, respectively

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Human TFF3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TFF3 Biotinylated Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human TFF3 Biotinylated Antibody

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