Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TFF3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Ala21-Phe80
Accession # Q07654

Specificity

Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TFF3 Antibody

Detection of Human TFF3 antibody by Western Blot.

Detection of Human TFF3 by Western Blot.

Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TFF3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TFF3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human pancreas tissue and KATO‑III human gastric carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TFF3

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22-80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30-72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29-80. Over aa 21-80, human TFF3 shares 77%, 68%, and 72% aa identity with mouse, canine, and rat TFF3, respectively.

Long Name

Trefoil Factor 3

Alternate Names

ITF, TFI

Entrez Gene IDs

7033 (Human); 21786 (Mouse); 25563 (Rat)

Gene Symbol

TFF3

UniProt

Q07654

Additional TFF3 Products

Product Documents for Human TFF3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human TFF3 Antibody

For research use only

Related Research Areas

Inflammatory Mediators

Citations for Human TFF3 Antibody

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