Human TFF3 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TFF3 by Western Blot.
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Product Details
Citations (1)
Supplemental Products

Human TFF3 Antibody Summary

Species Reactivity
Detects human Trefoil Factor 3 (TFF3) in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TFF1 and rhTFF2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TFF3
Accession # Q07654
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TFF3 antibody by Western Blot. View Larger

Detection of Human TFF3 by Western Blot. Western blot shows lysates of human pancreas tissue and KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TFF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4407) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TFF3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF3

TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. Mature human TFF3 is 59 amino acids (aa) in length (aa 22-80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30-72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N‑terminal extension and a third shows a 109 aa substitution for aa 29-80. Over aa 21-80, human TFF3 shares 77%, 68%, and 72% aa identity with mouse, canine, and rat TFF3, respectively.

Long Name
Trefoil Factor 3
Entrez Gene IDs
7033 (Human); 21786 (Mouse); 25563 (Rat)
Alternate Names
HITF, human intestinal trefoil factor10Intestinal trefoil factor; ITF; TFF3; TFI; trefoil factor 3 (intestinal)

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Citation for Human TFF3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Trefoil Factor 3 (TFF3) Is Regulated by Food Intake, Improves Glucose Tolerance and Induces Mucinous Metaplasia.
    Authors: Ge H, Gardner J, Wu X, Rulifson I, Wang J, Xiong Y, Ye J, Belouski E, Cao P, Tang J, Lee K, Coberly S, Wu X, Gupte J, Miao L, Yang L, Nguyen N, Shan B, Yeh W, Veniant M, Li Y, Baribault H
    PLoS ONE, 2015;10(6):e0126924.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development


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