Human TGF-beta  RII Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF241
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human TGF-beta  RII Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TGF-beta  RII in ELISAs and Western blots. In sandwich immunoassays, approximately 25% cross-reactivity with recombinant human (rh) TGF-beta RIIb is observed and less than 0.05% cross-reactivity with rhTGF-beta RI, rhTGF-beta RIII, recombinant mouse TGF-beta RII, and rhTGF-beta 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TGF‑ beta  RII
Ile24-Asp159
Accession # P37173
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TGF-beta  RII (Catalog # 241-R2)

Human TGF-beta RII Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human TGF‑ beta  RII Antibody (Catalog # AF-241-NA)

Standard: Recombinant Human TGF-beta RII Protein (Catalog # 241-R2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated type I (53 kDa), type II (70 - 85 kDa), and type III (250 - 350 kDa). The type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the type I and type II receptors.

The recombinant soluble TGF-beta type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References
  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.
Long Name
Transforming Growth Factor beta Receptor II
Entrez Gene IDs
7048 (Human); 21813 (Mouse)
Alternate Names
AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor type II

Product Datasheets

Citations for Human TGF-beta  RII Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Ad5/48 hexon oncolytic virus expressing sTGFbetaRIIFc produces reduced hepatic and systemic toxicities and inhibits prostate cancer bone metastases.
    Authors: Xu W, Zhang Z, Yang Y, Hu Z, Wang C, Morgan M, Wu Y, Hutten R, Xiao X, Stock S, Guise T, Prabhakar B, Brendler C, Seth P
    Mol Ther, 2014;22(8):1504-17.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  2. Ig-reactive CD4+CD25+ T cells from tolerized (New Zealand Black x New Zealand White)F1 mice suppress in vitro production of antibodies to DNA.
    Authors: La Cava A, Ebling FM, Hahn BH
    J. Immunol., 2004;173(5):3542-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human TGF-beta  RII Biotinylated Antibody

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Human TGF-beta  RII Biotinylated Antibody
By Anonymous on 10/16/2019
Application: ELISA Sample Tested: Recombinant protein Species: Human

Human TGF-beta  RII Biotinylated Antibody
By Anonymous on 05/07/2019
Application: WB Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse