Human Thrombospondin-4 Biotinylated Antibody

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Human Thrombospondin-4 Biotinylated Antibody Summary

Species Reactivity
Detects human Thrombospondin-4 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) THBS1, rhTHBS2, and rhTHBS3 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Thrombospondin‑4 (Catalog # 2390-TH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).

  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.
Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

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