Human TIM-4 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TIM-4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 921832
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TIM-4
    Accession # Q96H15
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TIM‑4 in HEK Human Cell Line Transfected with Human TIM-4 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TIM-4 and eGFP was stained with and either (A) Mouse Anti-Human TIM‑4 APC‑conjugated Monoclonal Antibody (Catalog # FAB2929A) or
(B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

  • References:
    1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
    2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. 
    3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
    4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
    5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
    6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
    7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
    8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
  • Long Name:
    T Cell Immunoglobulin Mucin-4
  • Entrez Gene IDs:
    91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)
  • Alternate Names:
    FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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