Human TIMP-4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF974
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Human TIMP-4 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIMP-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP-1, rhTIMP-2, rhTIMP-3, and recombinant mouse TIMP-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-4
Cys30-Pro224
Accession # Q99727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TIMP‑4 (Catalog # 974-TSF)

Human TIMP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human TIMP‑4 Antibody (Catalog # MAB974)

Standard: Recombinant Human TIMP-4 (Sf 21-expressed) Protein, CF (Catalog # 974-TSF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3 and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3 - 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

References
  1. Greene, et al. (1996) J. Biol. Chem. 271:30375.
  2. Leco, et al. (1997) FEBS Lett. 401:213.
  3. Geliker, et al. (2001) Oncogene 20:4337.
  4. Groft, et al. (2001) Br. J. Cancer 85:55.
  5. Hurst, et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
  6. Bigg, et al. (1997) J. Biol. Chem. 272:15496.
  7. Hernandez-Barrantes, et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
  8. Amour, et al. (1998) FEBS Lett. 435:39.
  9. Liu, et al. (1997) J. Biol. Chem. 272:20479.
Long Name
Tissue Inhibitors of Metalloproteinases 4
Entrez Gene IDs
7079 (Human); 110595 (Mouse)
Alternate Names
metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4

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