Human TIMP-4 Biotinylated Antibody

BAF974 Datasheet / COA / SDS

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BAF974

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Human TIMP-4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TIMP-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP-1, rhTIMP-2, rhTIMP-3, and recombinant mouse TIMP-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TIMP-4
Cys30-Pro224
Accession # Q99727

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TIMP‑4 (Catalog # 974-TSF)

Human TIMP-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human TIMP‑4 Antibody (Catalog # MAB974)

Standard: Recombinant Human TIMP-4 (Sf 21-expressed) Protein, CF (Catalog # 974-TSF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3 and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3 - 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

References

Greene, et al. (1996) J. Biol. Chem. 271:30375.
Leco, et al. (1997) FEBS Lett. 401:213.
Geliker, et al. (2001) Oncogene 20:4337.
Groft, et al. (2001) Br. J. Cancer 85:55.
Hurst, et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
Bigg, et al. (1997) J. Biol. Chem. 272:15496.
Hernandez-Barrantes, et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
Amour, et al. (1998) FEBS Lett. 435:39.
Liu, et al. (1997) J. Biol. Chem. 272:20479.

Long Name

Tissue Inhibitors of Metalloproteinases 4

Entrez Gene IDs

7079 (Human); 110595 (Mouse)

Alternate Names

metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4