Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3‑5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9). Human TIMP-4 shares 89% sequence indentity with Mouse TIMP-4.
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Key Product Details
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Label
Antibody Source
Product Specifications
Immunogen
Cys30-Pro224
Accession # Q99727
Specificity
Clonality
Host
Isotype
Scientific Data Images for TIMP-4 Antibody
Human TIMP-4 ELISA Standard Curve
Recombinant Human TIMP‑4 (Catalog # 974-TSF) was serially diluted and captured by Mouse Anti-Human TIMP‑4 Monoclonal Antibody (Catalog # MAB974) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF974) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for TIMP-4 Antibody
ELISA
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TIMP‑4 Monoclonal Antibody (Catalog # MAB7667). In sandwich immunoassays, this antibody is specific for mouse TIMP-4 when paired with the suggested capture antibody.
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TIMP-4 DuoSet ELISA Kit (Catalog # DY7667-05) for convenient development of a sandwich ELISA.
Western Blot
Sample: Recombinant Human TIMP‑4 (Catalog # 974-TSF) and Recombinant Mouse TIMP-4 (Catalog # 7667-TM)
Reviewed Applications
Read 1 review rated 4 using AF974 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TIMP-4
References
- Greene, et al. (1996) J. Biol. Chem. 271:30375.
- Leco, et al. (1997) FEBS Lett. 401:213.
- Geliker, et al. (2001) Oncogene 20:4337.
- Groft, et al. (2001) Br. J. Cancer 85:55.
- Hurst, et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
- Bigg, et al. (1997) J. Biol. Chem. 272:15496.
- Hernandez-Barrantes, et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
- Amour, et al. (1998) FEBS Lett. 435:39.
- Liu, et al. (1997) J. Biol. Chem. 272:20479.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TIMP-4 Products
Product Documents for TIMP-4 Antibody
Certificate of Analysis
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Product Specific Notices for TIMP-4 Antibody
For research use only
Related Research Areas
Citations for TIMP-4 Antibody
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Application: Western BlotSample Tested: Human cell conditioned mediumSpecies: HumanVerified Customer | Posted 02/27/2016
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting Guide
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