Human/Mouse TIMP-4 Antibody

Catalog # Availability Size / Price Qty
AF974
AF974-SP
Product Details
Citations (1)
FAQs
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Reviews (1)

Human/Mouse TIMP-4 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse TIMP‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TIMP-1, rhTIMP-2, and rhTIMP-3 is observed. In sandwich immunoassays, this antibody is specific for mouse TIMP-4 when paired with the suggested capture antibody.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-4
Cys30-Pro224
Accession # Q99727
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human TIMP‑4 (Catalog # 974-TSF) and Recombinant Mouse TIMP-4 (Catalog # 7667-TM)
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TIMP‑4 Monoclonal Antibody (Catalog # MAB7667). In sandwich immunoassays, this antibody is specific for mouse TIMP-4 when paired with the suggested capture antibody.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse TIMP-4 DuoSet ELISA Kit (Catalog # DY7667-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-4

Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3, and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3‑5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9). Human TIMP-4 shares 89% sequence indentity with Mouse TIMP-4. 

References
  1. Greene, et al. (1996) J. Biol. Chem. 271:30375.
  2. Leco, et al. (1997) FEBS Lett. 401:213.
  3. Geliker, et al. (2001) Oncogene 20:4337.
  4. Groft, et al. (2001) Br. J. Cancer 85:55.
  5. Hurst, et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
  6. Bigg, et al. (1997) J. Biol. Chem. 272:15496.
  7. Hernandez-Barrantes, et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
  8. Amour, et al. (1998) FEBS Lett. 435:39.
  9. Liu, et al. (1997) J. Biol. Chem. 272:20479.
Long Name
Tissue Inhibitors of Metalloproteinases 4
Entrez Gene IDs
7079 (Human); 110595 (Mouse)
Alternate Names
metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4

Product Datasheets

Citation for Human/Mouse TIMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
    Authors: Ooi YH, Oh DJ, Rhee DJ
    Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse TIMP‑4 Antibody
By Anonymous on 02/27/2016
Application: WB Sample Tested: Human cell conditioned medium Species: Human