Human TLR1 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human TLR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant mouse (rm) TLR1 is observed and less than 5% cross-reactivity with recombinant human (rh) TLR3, rhTLR4, and rmTLR6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TLR1
    Accession # AAC34137
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR1 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Goat Anti-Human TLR1 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1484A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR1

The Toll-like family of molecules are type I transmembrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human TLRs that activate the innate immune system following exposure to a variety of microbial species (1, 2). TLRs contain a large number of leucine-rich repeats (LRRs) and a cytoplasmic tail with one Toll/IL-1 receptor (TIR) domain. Mature human TLR1 consists of a 556 amino acid (aa) extracellular domain (ECD) with 20 LRRs, a 21 aa transmembrane segment, and a 185 aa cytoplasmic domain (3, 4). Within the ECD, human TLR1 shares 63% aa sequence identity with human TLR6 and 20%‑43% aa sequence identitity with human TLR2, -3, -4, -5, -7, -8, -9, and -10. It shares 73% and 71% aa sequence identity with mouse and rat TLR1, respectively. TLR1 is expressed on the surface of macrophages, dendritic cells, and tonsillar epithelial cells in ligand-independent association with TLR2 (5‑8). TLR2 additionally associates with TLR6 to form a functional complex with specificity for distinct but related microbial ligands (9‑11). TLR1 and TLR2 cooperate in the recognition of bacterial and protozoal triacylated lipopeptides and glycosylphosphatidylinositols (6, 10‑12). Ligand binding induces TLR1 localization to lipid rafts followed by receptor internalization and activation of NF kappa B (7, 11, 13).

  • References:
    1. Miyake, K. (2007) Semin. Immunol. 19:3.
    2. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    3. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
    4. Matsushima, N. et al. (2007) BMC Genomics 8:124.
    5. Ochoa, M.-T. et al. (2003) Immunology 108:10.
    6. Takeuchi, O. et al. (2002) J. Immunol. 169:10.
    7. Triantafilou, M. et al. (2006) J. Biol. Chem. 281:31002.
    8. Sandor, F. et al. (2003) J. Cell Biol. 162:1099.
    9. Nakao, Y. et al. (2005) J. Immunol. 174:1566.
    10. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. 97:13766.
    11. Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
    12. Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
    13. Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.
  • Long Name:
    Toll-like Receptor 1
  • Entrez Gene IDs:
    7096 (Human); 21897 (Mouse); 305354 (Rat)
  • Alternate Names:
    CD281 antigen; CD281; DKFZp547I0610; DKFZp564I0682; KIAA0012MGC126311; MGC104956; MGC126312; rsc786; TIL; TIL. LPRS5; Toll/interleukin-1 receptor-like protein; toll-like receptor 1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. A Common Genetic Variant in TLR1 Enhances Human Neutrophil Priming and Impacts Length of Intensive Care Stay in Pediatric Sepsis.
    Authors: Whitmore L, Hook J, Philiph A, Hilkin B, Bing X, Ahn C, Wong H, Ferguson P, Moreland J
    J Immunol, 2016;196(3):1376-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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