Human TLR3 Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (9)
Supplemental Products

Human TLR3 Antibody Summary

Species Reactivity
Detects human TLR3 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human TLR3
Accession # O15455
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human TLR3 (Catalog # 1487-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8 - 11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11 - 13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13 - 15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

  1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
  2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
  3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  4. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
  5. Choe, J. et al. (2005) Science 309:581.
  6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
  7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
  8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
  9. Alexopoulou, L. et al. (2001) Nature 413:732.
  10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
  11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
  12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
  13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
  14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
  15. Wang, T. et al. (2004) Nat. Med. 10:1366.
Long Name
Toll-like Receptor 3
Entrez Gene IDs
7098 (Human); 142980 (Mouse); 364594 (Rat)
Alternate Names
CD283 antigen; CD283; TLR3; toll-like receptor 3

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Citations for Human TLR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Increased expression of toll-like receptor 3, an anti-viral signaling molecule, and related genes in Alzheimer's disease brains.
    Authors: Walker D, Tang T, Lue L
    Exp Neurol, 2018;309(0):91-106.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Surface Toll-Like Receptor 3 Expression in Metastatic Intestinal Epithelial Cells induces Inflammatory Cytokine Production and Promotes Invasiveness
    Authors: M Bugge, B Bergstrom, OK Eide, H Solli, IF Kjønstad, J Stenvik, T Espevik, NJ Nilsen
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5
    PLoS ONE, 2016;11(8):e0160875.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Proteolytic processing regulates Toll-like receptor 3 stability and endosomal localization.
    Authors: Qi, Rongsu, Singh, Divyendu, Kao, C Cheng
    J Biol Chem, 2012;287(39):32617-29.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  5. LL37 and cationic peptides enhance TLR3 signaling by viral double-stranded RNAs.
    Authors: Lai Y, Adhikarakunnathu S, Bhardwaj K
    PLoS ONE, 2011;6(10):e26632.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Herpes simplex virus encephalitis in a patient with complete TLR3 deficiency: TLR3 is otherwise redundant in protective immunity.
    Authors: Guo Y, Audry M, Ciancanelli M, Alsina L, Azevedo J, Herman M, Anguiano E, Sancho-Shimizu V, Lorenzo L, Pauwels E, Philippe PB, Perez de Diego R, Cardon A, Vogt G, Picard C, Andrianirina ZZ, Rozenberg F, Lebon P, Plancoulaine S, Tardieu M, Valérie Doireau E, Jouanguy D, Chaussabel F, Geissmann L, Abel JL, Casanova SY, Zhang
    J. Exp. Med., 2011;208(10):2083-98.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. Viral double-strand RNA-binding proteins can enhance innate immune signaling by toll-like Receptor 3.
    Authors: Lai Y, Yi G, Chen A
    PLoS ONE, 2011;6(10):e25837.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Oxidative stress enhances toll-like receptor 3 response to double-stranded RNA in airway epithelial cells.
    Authors: Koarai A, Sugiura H, Yanagisawa S
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):651-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Activation of Toll-like receptor 3 augments myofibroblast differentiation.
    Authors: Sugiura H, Ichikawa T, Koarai A, Yanagisawa S, Minakata Y, Matsunaga K, Hirano T, Akamatsu K, Ichinose M
    Am. J. Respir. Cell Mol. Biol., 2009;40(6):654-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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