Human TLR3 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human TLR3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR4 and recombinant mouse TLR6 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TLR3
    Accession # O15455
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of TLR3 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human TLR3 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1487A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 Receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8-11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11-13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13-15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

  • References:
    1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
    2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
    3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
    4. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
    5. Choe, J. et al. (2005) Science 309:581.
    6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
    7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
    8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
    9. Alexopoulou, L. et al. (2001) Nature 413:732.
    10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
    11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
    12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
    13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
    14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
    15. Wang, T. et al. (2004) Nat. Med. 10:1366.
  • Long Name:
    Toll-like Receptor 3
  • Entrez Gene IDs:
    7098 (Human); 142980 (Mouse); 364594 (Rat)
  • Alternate Names:
    CD283 antigen; CD283; toll-like receptor 3
Related Research Areas
Isotype Controls
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Goat IgG APC-conjugated Antibody

Ctrl IC108A 2  
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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