Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
View all TLR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TLR3
Lys27-Ser711
Accession # O15455

Specificity

Detects human TLR3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TLR1, rhTLR2, rhTLR4 and recombinant mouse TLR6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TLR3 APC‑conjugated Antibody

Detection of TLR3 antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of TLR3 in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Goat Anti-Human TLR3 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1487A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human TLR3 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: A549 human lung carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 2 reviews rated 3.5 using IC1487A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR3

Human TLR3 is a 116 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules (1, 2). There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (3). The human TLR3 cDNA encodes a 904 amino acid (aa) precursor that contains a 23 aa signal sequence, a 681 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 179 aa cytoplasmic region (4). The horseshoe shaped ECD (5, 6) contains 23 leucine rich repeats, and the cytoplasmic domain contains one Toll/IL-1 Receptor (TIR) domain. The ECD of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively. TLR3 is found in phagosomes (7), where the acidic pH enables binding of internalized double stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells (8-11). Ligand binding by TLR3 induces receptor dimerization (5, 6, 8) leading to the release of inflammatory cytokines and dendritic cell maturation (9, 11-13). TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes (13-15) and is upregulated by IFN-beta and LPS (9, 14). TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection (12).

References

  1. Sen, G.C. and S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1.
  2. Schroder, M. and A.G. Bowie (2005) Trends Immunol. 26:462.
  3. Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
  4. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. 95:588.
  5. Choe, J. et al. (2005) Science 309:581.
  6. Bell, J.K. et al. (2005) Proc. Natl. Acad. Sci. 102:10976.
  7. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107.
  8. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133.
  9. Alexopoulou, L. et al. (2001) Nature 413:732.
  10. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277.
  11. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542.
  12. Rudd, B.D. et al. (2005) J. Virol. 79:3350.
  13. Farina, C. et al. (2005) J. Neuroimmunol. 159:12.
  14. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502.
  15. Wang, T. et al. (2004) Nat. Med. 10:1366.

Long Name

Toll-like Receptor 3

Alternate Names

CD283

Entrez Gene IDs

7098 (Human); 142980 (Mouse); 364594 (Rat)

Gene Symbol

TLR3

UniProt

O15455

Additional TLR3 Products

Product Documents for Human TLR3 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human TLR3 APC‑conjugated Antibody

For research use only

Customer Reviews for Human TLR3 APC‑conjugated Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human TLR3 APC-conjugated Antibody
    Name: Stephane Hua
    Application: Flow Cytometry
    Sample Tested: Dendritic cells
    Species: Human
    Verified Customer | Posted 07/05/2018
  • Human TLR3 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human peripheral CD11c+ dendritic cells and BDCA3 dendritic cells
    Species: Human
    Verified Customer | Posted 06/24/2018

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