Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.
Monoclonal Mouse IgG2A Clone # 610015
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TLR4 Glu24-Lys631 Accession # O00206
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of TLR4 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human TLR4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB6248C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.
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