Human TLR4 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 610015
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TLR4
    Accession # O00206
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TLR4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TLR4 PE‑conjugated Monoclonal Antibody (Catalog # FAB6248P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TLR4

Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

  • Long Name:
    Toll-like Receptor 4
  • Entrez Gene IDs:
    7099 (Human); 21898 (Mouse); 29260 (Rat)
  • Alternate Names:
    ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TOLL; toll-like receptor 4
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015;194(8):3917-23.
    Species: Human
    Sample Type: Whole Blood
    Application: Flow
  2. Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
    Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
    PLoS ONE, 2013;8(12):e82734.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 10  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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