Human TLR4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6248P
Human TLR4 PE-conjugated Antibody in Data
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Product Details
Citations (6)
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Human TLR4 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TLR1, 2, 3, 5, 7, 8, 9, 10, recombinant mouse (rm) TLR4, rmTLR6, rhTIRAP, rhIL-1 RI, rhIL-1 RII, rhIL-1 RAcP, rhST2, rhIL-18 R, rhIL-1 Rrp2, rhIL-18 R beta, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhMD-1, rhMD-2, or rmRP105 is observed.
Source
Monoclonal Mouse IgG2A Clone # 610015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TLR4
Glu24-Lys631
Accession # O00206
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TLR4 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of TLR4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TLR4 PE-conjugated Monoclonal Antibody (Catalog # FAB6248P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR4

Toll-Like Receptor (TLR4) belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name
Toll-like Receptor 4
Entrez Gene IDs
7099 (Human); 21898 (Mouse); 29260 (Rat)
Alternate Names
ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Product Datasheets

Citations for Human TLR4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Phenotypic and functional features of innate and adaptive immunity as putative biomarkers for clinical status and leprosy reactions
    Authors: J Campos de, MG Araújo, JG Alves Coel, V Peruhype-M, CC Alvares, M de Lima Mo, A Teixeira-C, OA Martins-Fi, MS Silva Araú
    Microb. Pathog., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Phagocytosis and Inflammation: Exploring the effects of the components of E-cigarette vapor on macrophages
    Authors: MP Ween, JJ Whittall, R Hamon, PN Reynolds, SJ Hodge
    Physiol Rep, 2017;5(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015;194(8):3917-23.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  4. Serum amyloid A in the placenta and its role in trophoblast invasion.
    Authors: Sandri S, Urban Borbely A, Fernandes I, de Oliveira E, Knebel F, Ruano R, Zugaib M, Filippin-Monteiro F, Bevilacqua E, Campa A
    PLoS ONE, 2014;9(3):e90881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2014;133(1):172-80.e1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Chip Cytometry
  6. Ex vivo cytokine release and pattern recognition receptor expression of subjects exposed to dampness: pilot study to assess the outcome of mould exposure to the innate immune system.
    Authors: Punsmann S, Liebers V, Lotz A, Bruning T, Raulf M
    PLoS ONE, 2013;8(12):e82734.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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