Human TLR5 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6704G
Detection of TLR5 in Human PBMC by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human TLR5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP is observed.
Source
Monoclonal Mouse IgG1 Clone # 624915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TLR5
Ile21-Phe115
Accession # O60602
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TLR5 in Human PBMC by Flow Cytometry. View Larger

Detection of TLR5 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Mouse Anti-Human TLR5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6704G) or (B) isotype control antibody (Catalog # IC002G) and Mouse anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TLR5

TLR5 is an ~100 kDa type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% amino acid identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88.

Long Name
Toll-like Receptor 5
Entrez Gene IDs
7100 (Human); 53791 (Mouse); 289337 (Rat)
Alternate Names
MGC126430; MGC126431; SLEB1FLJ10052; TIL-3; TIL3Toll/interleukin-1 receptor-like protein 3; TLR5; toll-like receptor 5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human TLR5 Alexa Fluor® 488-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human TLR5 Alexa Fluor® 488-conjugated Antibody and earn rewards!

Have you used Human TLR5 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review