Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with
recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or
rhTIRAP is observed.
Monoclonal Mouse IgG1 Clone # 624915
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human TLR5 Ile21-Phe115 Accession # O60602
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TLR5 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TLR5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6704G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
TLR5 is an ~100 kDa type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% amino acid identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.