Human TLR5 Antibody

Catalog # Availability Size / Price Qty
MAB6704
MAB6704-SP
Human TLR5 Antibody in Immunohistochemistry
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Human TLR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP is observed.
Source
Monoclonal Mouse IgG1 Clone # 624915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TLR5
Ile21-Phe115
Accession # O60602
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry TLR5 in Human Intestine. View Larger

TLR5 in Human Intestine. TLR5 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panels show a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry Detection of TLR5 in Human Human Peripheral Blood Monocytes by Flow Cytometry. View Larger

Detection of TLR5 in Human Human Peripheral Blood Monocytes by Flow Cytometry. Human Human Peripheral Blood Monocytes were stained with (A) Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) or (B) isotype control antibody (Catalog # MAB002), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR5

TLR5 is an ~100 kDa type I transmembrane glycoprotein of the Toll-like receptor family. It is expressed on mucosal epithelia in the gastrointestinal tract, airways, and other areas of potential bacterial contact and recognizes bacterial flagellin. Expression is also reported on monocytes, immature dendritic cells, and CD4+ T lymphocytes. The region of human TLR5 used as an immunogen includes the first 2 of 16 leucine-rich repeats and shares 72% amino acid identity with mouse and rat TLR5. This region is outside of the flagellin binding region. Flagellin engagement induces dimerization and intracellular signaling via MyD88.

Long Name
Toll-like Receptor 5
Entrez Gene IDs
7100 (Human); 53791 (Mouse); 289337 (Rat)
Alternate Names
MGC126430; MGC126431; SLEB1FLJ10052; TIL-3; TIL3Toll/interleukin-1 receptor-like protein 3; TLR5; toll-like receptor 5

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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