TMEM219 (Transmembrane protein 219; also IGFBP3 Receptor) is a 30-35 kDa member of the 2-transmembrane group of the Pfam-A family of polytopic molecules. It is widely expressed, and appears to be a cell-surface receptor for IGFBP3. Only free IGFBP3 will bind to TMEM219; when complexed to IGF-I, IGFBP3 is unable to recognize the TMEM219 molecule. Ligation of TMEM219 inhibits cell proliferation and induces caspase-8 mediated apoptosis. It is suggested that procaspase-8 is normally sequestered by TMEM219. Following IGFBP3 binding, procaspase-8 is activated and released from TMEM219, resulting in the downstream activation of executioner caspases. Human TMEM219 is a 240 amino acid (aa) 2-transmembrane protein. It contains an N-terminal 20 aa cytoplasmic region, a 21 aa TM domain, a 164 aa extracellular region (aa 41-204), a second 21 aa TM domain, and a C-terminal 15 aa cytoplasmic tail. Over aa 41-200, human TMEM219 shares 82% aa sequence identity with mouse TMEM219.
Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody
R&D Systems | Catalog # AF7556AFP680
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Specificity
Clonality
Host
Isotype
Applications for Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody
Immunohistochemistry
Western Blot
Formulation, Preparation, and Storage
Formulation
Shipping
Stability & Storage
Background: TMEM219
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TMEM219 Products
Product Documents for Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Related Research Areas
Customer Reviews
There are currently no reviews for this product. Be the first to review Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody and earn rewards!
Have you used Human TMEM219 Alexa Fluor™ Plus 680‑conjugated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars