Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TMEM219 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TMEM219
Leu41-Leu200
Accession # ACQ72822

Specificity

Detects human TMEM219 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TMEM219 Antibody

Detection of Human TMEM219 antibody by Western Blot.

Detection of Human TMEM219 by Western Blot.

Western blot shows lysates of human liver tissue, human skeletal muscle tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TMEM219 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TMEM219 antibody in Human Prostate Gland by Immunohistochemistry (IHC-P).

TMEM219 in Human Prostate Gland.

TMEM219 was detected in immersion fixed paraffin-embedded sections of human prostate gland using Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in stromal cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human TMEM219 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate gland

Western Blot

1 µg/mL
Sample: Human liver tissue, human skeletal muscle tissue, and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEM219

TMEM219 (Transmembrane protein 219; also IGFBP3 Receptor) is a 30-35 kDa member of the 2-transmembrane group of the Pfam-A family of polytopic molecules. It is widely expressed, and appears to be a cell-surface receptor for IGFBP3. Only free IGFBP3 will bind to TMEM219; when complexed to IGF-I, IGFBP3 is unable to recognize the TMEM219 molecule. Ligation of TMEM219 inhibits cell proliferation and induces caspase-8 mediated apoptosis. It is suggested that procaspase-8 is normally sequestered by TMEM219. Following IGFBP3 binding, procaspase-8 is activated and released from TMEM219, resulting in the downstream activation of executioner caspases. Human TMEM219 is a 240 amino acid (aa) 2-transmembrane protein. It contains an N-terminal 20 aa cytoplasmic region, a 21 aa TM domain, a 164 aa extracellular region (aa 41-204), a second 21 aa TM domain, and a C-terminal 15 aa cytoplasmic tail. Over aa 41-200, human TMEM219 shares 82% aa sequence identity with mouse TMEM219.

Long Name

Transmembrane Protein 219

Alternate Names

IGFBP3R, LOC124446

Entrez Gene IDs

124446 (Human); 68742 (Mouse)

Gene Symbol

TMEM219

UniProt

ACQ72822

Additional TMEM219 Products

Product Documents for Human TMEM219 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TMEM219 Antibody

For research use only

Citations for Human TMEM219 Antibody

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Protocols

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