Detection of Human TMEM219 by Western Blot. Western blot shows lysates of human liver tissue, human skeletal muscle tissue, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TMEM219 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7556) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TMEM219 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TMEM219 (Transmembrane protein 219; also IGFBP3 Receptor) is a 30-35 kDa member of the 2-transmembrane group of the Pfam-A family of polytopic molecules. It is widely expressed, and appears to be a cell-surface receptor for IGFBP3. Only free IGFBP3 will bind to TMEM219; when complexed to IGF-I, IGFBP3 is unable to recognize the TMEM219 molecule. Ligation of TMEM219 inhibits cell proliferation and induces caspase-8 mediated apoptosis. It is suggested that procaspase-8 is normally sequestered by TMEM219. Following IGFBP3 binding, procaspase-8 is activated and released from TMEM219, resulting in the downstream activation of executioner caspases. Human TMEM219 is a 240 amino acid (aa) 2-transmembrane protein. It contains an N-terminal 20 aa cytoplasmic region, a 21 aa TM domain, a 164 aa extracellular region (aa 41-204), a second 21 aa TM domain, and a C-terminal 15 aa cytoplasmic tail. Over aa 41-200, human TMEM219 shares 82% aa sequence identity with mouse TMEM219.
Transmembrane Protein 219
Entrez Gene IDs:
124446 (Human); 68742 (Mouse)
IGFBP3R; IGFBP-3R; Insulin-Like Growth Factor Binding Protein-3 Receptor; Insulin-Like Growth Factor-Binding Protein 3 Receptor; LOC124446; TMEM219; Transmembrane Protein 219
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