Human TMIGD2/CD28H PE-conjugated Antibody

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Human TMIGD2/CD28H PE-conjugated Antibody in Flow Cytometry
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Product Details
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Human TMIGD2/CD28H PE-conjugated Antibody Summary

Species Reactivity
Detects human TMIGD2/CD28H in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 953743
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK-293-derived human TMIGD2/CD28H
Accession # Q96BF3
Supplied in a saline solution containing BSA and Sodium Azide.


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry. View Larger

Detection of TMIGD2/CD28H in HEK293 Human Cell Line Transfected with Human TMIGD2/CD28H and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H PE-conjugated Monoclonal Antibody (Catalog # FAB83162P) or (B) Mouse IgG2A Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
Long Name
Transmembrane And Immunoglobulin Domain Containing 2
Entrez Gene IDs
126259 (Human)
Alternate Names
CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2

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