Human TMIGD2/CD28H Antibody

Catalog # Availability Size / Price Qty
MAB83162-100
MAB83162-SP
Detection ofTMIGD2/CD28H in HEK293 Human Cell Line Transfected with HumanTMIGD2/CD28H and eGFP by Flow Cytometry.
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Human TMIGD2/CD28H Antibody Summary

Species Reactivity
Human
Specificity
Detects human TMIGD2/CD28H in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 953743
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK-293-derived human TMIGD2/CD28H
Met1-Gly150
Accession # Q96BF3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functionalflow cytometry test, 50 μg/mL of Mouse Anti-Human TMIGD2/CD28H Antibody(Catalog # MAB83162) will block the binding of 500 ng/mL Recombinant Human B7-H7Fc Chimera (Catalog #156-B7)to HEK293 human embryonic kidney cell line transfected with recombinant human TMIGD2/CD28H.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection ofTMIGD2/CD28H in HEK293 Human Cell Line Transfected with HumanTMIGD2/CD28H and eGFP by Flow Cytometry. View Larger

Detection ofTMIGD2/CD28H in HEK293 Human Cell Line Transfected with HumanTMIGD2/CD28H and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human TMIGD2/CD28H and eGFP was stained with and either (A) Mouse Anti-Human TMIGD2/CD28H Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Blockade of Receptor-ligand Interaction B7-H7binding to TMIGD2/CD28H-transfected HEK293 Human Cell Line is Blocked byHuman TMIGD2/CD28H Antibody. View Larger

B7-H7binding to TMIGD2/CD28H-transfected HEK293 Human Cell Line is Blocked byHuman TMIGD2/CD28H Antibody. In a functional flow cytometry test,Recombinant Human
B7-H7 Fc Chimera (Catalog #156-B7,
500 ng/mL) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human TMIGD2/CD28H (black dotted line). Binding iscompletely blocked (orange histogram) by 50 μg/mL of Mouse Anti-Human TMIGD2/CD28HMonoclonal Antibody (Catalog # MAB83162). Mouse IgG2B Isotype Control(Catalog #MAB003)at 50 μg/mL was used as a control (blue line). Cells were stained with MouseAnti-Human IgG1 Fc APC‑conjugated Monoclonal Antibody (Catalog #FAB110A).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMIGD2/CD28H

CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).

References
  1. Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
  2. Parry, R.V. et al. (2003) J.Immunol. 171:166.
  3. Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
  4. Huang, C. et al. (2003) Nature 424:219.
  5. Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
Long Name
Transmembrane And Immunoglobulin Domain Containing 2
Entrez Gene IDs
126259 (Human)
Alternate Names
CD28H; IGPR1; IGPR-1; MGC23244; TMIGD2; transmembrane and immunoglobulin domain containing 2; transmembrane and immunoglobulin domain-containing protein 2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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