Human Total IL-18/IL-1F4 Quantikine ELISA Kit

  
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  • Assay Length
    4.0 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (17 uL), EDTA Plasma (17 uL), Heparin Plasma (17 uL), Saliva (25 uL), Urine (50 uL)
  • Sensitivity
    5.15 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
  • Specificity
    Natural and recombinant human Total IL-18.
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC231 , Quantikine Immunoassay Control Set 919 for Human Total IL-18 - Please Inquire
Product Summary
The Quantikine® Human Total IL-18/IL-1F4 Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IL-18 in cell culture supernates, serum, plasma, saliva, and urine. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 126 251 495 126 248 494
Standard Deviation 3.85 6.33 14.6 10.9 19.7 41.6
CV% 3.1 2.5 2.9 8.7 7.9 8.4

Cell Culture Supernates, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 175 301 636 197 381 739
Standard Deviation 5.49 8.06 24.6 27.4 46.7 81.1
CV% 3.1 2.7 3.9 13.9 12.3 11

Recovery

The recovery of human Total IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 90-102
EDTA Plasma (n=4) 104 93-113
Heparin Plasma (n=4) 102 88-110
Saliva (n=4) 108 94-120
Serum (n=4) 100 96-105
Urine (n=4) 91 84-100
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Total IL-18 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Total IL-18/IL-1F4 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-18/IL-1F4
Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.
  • Long Name:
    Interleukin 18
  • Entrez Gene IDs:
    3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
  • Alternate Names:
    Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18
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Quantikine ELISA Wash Buffer 1

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