Human Total IL-18 Quantikine QuicKit ELISA

Catalog # Availability Size / Price Qty
QK318
Control Products Available
Human IL-18 /IL-1F4 ELISA Cell Culture Supernate Standard Curve
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Human Total IL-18 Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
4.57 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-18.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human Total IL-18/IL-1F4 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IL-18 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-18 and has been shown to accurately quantitate the recombinant factor free and in complex with IL-18 BPa. Results obtained using natural human IL-18 showed linear curves that were parallel to the standard curves obtained using the QuicKit™ standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18.

Precision

Intra-Assay Precision (Precision within an assay) <div>Two samples of known concentration were tested twenty times on one plate to assess&nbsp;intra-assay precision.</div>
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technici

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean 120 591 131 638
Standard Deviation 4.23 22 6.9 38.1
CV% 3.5 3.7 5.3 6

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean 105 507 114 552
Standard Deviation 4.15 12.8 6.12 43.8
CV% 4 2.5 5.4 7.9

Recovery

The recovery of human IL-18 spiked to three different level throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 117 109-128
EDTA Plasma (n=2) 116 108-123
Heparin Plasma (n=2) 101 93-112
Serum (n=2) 101 85-117

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IL-18 were serially diluted with the appropriate calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-18/IL-1F4 ELISA Linearity

Data Examples

Human IL-18 /IL-1F4 ELISA Cell Culture Supernate Standard Curve

Human IL-18/IL-1F4 ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.

Long Name:
Interleukin 18
Entrez Gene IDs:
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names:
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

FAQs

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