TRA-1-81 in Human iPS Cells. TRA-1-81 was detected in immersion fixed human induced pluripotent stem (iPS) cells using Mouse Anti-Human TRA-1-81 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # IC8495T; red) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface
of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem
(ES), and induced pluripotent stem (iPS) cells. TRA-1-81 is an epitope expressed on podocalyxin,
also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I
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