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Human TRAIL/TNFSF10 PE-conjugated Antibody

  (3 citations)     
Datasheet / CoA / SDS
Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry.

Flow Cytometry

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Product Details
Citations (3)
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  • Species Reactivity
    Human
  • Specificity
    Detects human TRAIL in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 75402
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TRAIL
    Thr95-Gly281
    Accession # P50591
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets
Product Datasheet

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry TRAIL/TNFSF10 Antibody (75402) [Phycoerythrin]
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Detection of TRAIL/TNFSF10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs), untreated (A) or treated (B) with rhIFN- alpha for 48 hours (150 ng/mL), were stained with Mouse Anti-Human TRAIL/TNFSF10 PE‑conjugated Monoclonal Antibody (Catalog # FAB687P) and Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # 3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002P).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAIL/TNFSF10
TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.
  • Long Name:
    TNF-related Apoptosis-inducing Ligand
  • Entrez Gene IDs:
    8743 (Human); 22035 (Mouse)
  • Alternate Names:
    Apo-2 ligand; APO2L; Apo-2Ltumor necrosis factor (ligand) family, member 10; APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; CD253; Protein TRAIL; TL2; TNF-related apoptosis-inducing ligand; TNFSF10; TRAIL; TRAILTNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor ligand superfamily member 10
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
 Applications
 Sample Type
  1. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Extracellular HCV-core protein induces an immature regulatory phenotype in NK cells: implications for outcome of acute infection.
    Authors: Golden-Mason L, Hahn Y, Strong M, Cheng L, Rosen H
    PLoS ONE, 2014;9(7):e103219.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity.
    Authors: Kalb ML, Glaser A, Stary G, Koszik F, Stingl G
    J. Immunol., 2012;188(4):1583-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

 Ctrl IC002P 39  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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