Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Transglutaminase 4/TGM4 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4/TGM4
Met2-Lys684
Accession # AAC50516

Specificity

Detects human Transglutaminase 4 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant mouse Transglutaminase 2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Transglutaminase 4/TGM4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Transglutaminase 4/TGM4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transglutaminase 4/TGM4

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4.

Alternate Names

Fibrinoligase, TGM4, TGP

Entrez Gene IDs

7047 (Human); 331046 (Mouse); 64679 (Rat)

Gene Symbol

TGM4

UniProt

AAC50516

Additional Transglutaminase 4/TGM4 Products

Product Documents for Human Transglutaminase 4/TGM4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Transglutaminase 4/TGM4 Biotinylated Antibody

For research use only

Related Research Areas

Extracellular Matrix Molecules

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