Human TREM-1 Alexa Fluor® 488-conjugated Antibody

Clone 193015 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1278G
Human TREM-1 Alexa Fluor® 488-conjugated Antibody in Data
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Human TREM-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM-1 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 193015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Ala21-Asn205
Accession # Q9NP99
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below
Knockout Validated
TREM-1 is specifically detected in THP-1 myelogenous leukemia parental cell line but is not detectable in TREM-1 knockout THP-1 cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of TREM‑1 in THP-1Human Cell Line by Flow Cytometry. THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (IC002G). Staining was performed using our Staining Membrane-associated Proteins protocol.

Knockout Validated View Larger

TREM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. TREM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1278G, filled histogram) or isotype control antibody (IC002G,open histogram). No staining in the TREM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

Product Datasheets

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