Human TREM-1 PE-conjugated Antibody

Clone 193015 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB1278P
Human TREM-1 PE-conjugated Antibody in Data
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human TREM-1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TREM-1 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 193015
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TREM-1
Ala21-Asn205
Accession # Q9NP99
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
TREM‑1 is specifically detected in THP-1 human acute monocytic leukemia parental cell line but is not detectable in TREM‑1 knockout THP-1 cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of TREM‑1 in THP-1 Human Cell Line by Flow Cytometry THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Knockout Validated View Larger

TREM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line. TREM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P,open histogram). No staining in the TREM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

Product Datasheets

Citations for Human TREM-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells
    Authors: J Hyun, RS McMahon, AL Lang, JS Edwards, AD Badilla, ME Greene, GW Stone, S Pallikkuth, M Stevenson, DM Dykxhoorn, S Kottilil, S Pahwa, E Thomas
    PLoS Pathog., 2019;15(7):e1007883.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Activation and Regulation of Blood V?2 T Cells Are Amplified by TREM-1during Active Pulmonary Tuberculosis
    Authors: Y Wu, YM Fang, L Ding, X Liu, NM Francisco, J Wen, C Liao, Z Ma, Z Li, M Li, S Ming, T Liu, M Zhang, M Wu, M Jacobs, S Gong, X Huang
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Urine TREM-1 as a marker of urinary tract infection in children
    Authors: E Sierra-Dia, A Bravo Cuél, PC Ortiz Laza, M García Gut, HF Georgina, R Anaya Prad
    J. Int. Med. Res., 2017;45(2):631-638.
    Species: Human
    Sample Types: Urine
    Applications: Flow Cytometry
  4. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer
    Oncotarget, 2016;7(26):40508-40517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Differential pattern of cell-surface and soluble TREM-1 between sepsis and SIRS.
    Cytokine, 2013;61(1):112-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-kappaB pathway.
    J. Cell. Sci., 2012;91(6):933-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Hypoxia modulates the gene expression profile of immunoregulatory receptors in human mature dendritic cells: identification of TREM-1 as a novel hypoxic marker in vitro and in vivo.
    Authors: Bosco MC, Pierobon D, Blengio F, Raggi F, Vanni C, Gattorno M, Eva A, Novelli F, Cappello P, Giovarelli M, Varesio L
    Blood, 2011;117(9):2625-39.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Effects of TREM-1 activation in human neutrophils: activation of signaling pathways, recruitment into lipid rafts and association with TLR4.
    Authors: Fortin CF, Lesur O, Fulop T
    Int. Immunol., 2007;19(1):41-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Human TREM-1 PE-conjugated Antibody
By Anonymous on 06/24/2018
Application: Flow Sample Tested: Peripheral blood mononuclear cells (PBMCs),human CD4 T cells Species: Human

the expression TREM1 level was analyzed in CD4 T cells.