TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. The natural ligand for TREM-1 has not been identified. However, engagement of TREM-1 with an agonist monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha, suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock. Human and mouse TREM-1 share approximately 42% amino acid sequence homology (1-3).
Human TREM‑1 PE‑conjugated Antibody
R&D Systems | Catalog # FAB1278P
Key Product Details
Validated by
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Ala21-Asn205
Accession # Q9NP99
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human TREM‑1 PE‑conjugated Antibody
Detection of TREM‑1 in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TREM‑1 in THP-1 Human Cell Line by Flow Cytometry
THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
TREM-1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.
TREM-1 knockout THP-1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1278P, filled histogram) or isotype control antibody (IC002P,open histogram). No staining in the TREM-1 knockout THP-1 cell line was observed. Staining was performed using our Staining Membrane-associated Proteins protocol.
Applications for Human TREM‑1 PE‑conjugated Antibody
Flow Cytometry
Sample: Human peripheral blood granulocytes
Knockout Validated
Reviewed Applications
Read 1 review rated 5 using FAB1278P in the following applications:
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Advanced Features
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Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREM-1
References
- Bouchon, A. (2000) J. Immunol. 164:4991.
- Bouchon, A. (2001) Nature 410:1103.
- Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TREM-1 Products
Product Documents for Human TREM‑1 PE‑conjugated Antibody
Certificate of Analysis
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Product Specific Notices for Human TREM‑1 PE‑conjugated Antibody
For research use only
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Citations for Human TREM‑1 PE‑conjugated Antibody
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Application: Flow CytometrySample Tested: Peripheral blood mononuclear cells (PBMCs) and human CD4 T cellsSpecies: HumanVerified Customer | Posted 06/24/2018the expression TREM1 level was analyzed in CD4 T cells.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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