Human TREM2 Biotinylated Antibody
Human TREM2 Biotinylated Antibody Summary
His19-Ser174
Accession # Q9NZC2
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TREM2 in Human PBMCs. TREM2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS using Goat Anti-Human TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of TREM-2 in THP-1 Human Cell Line by Flow Cytometry. THP-1 human monocytic leukemia cell line was stained with Goat Anti-Human TREM-2 Biotinylated Polyclonal Antibody (Catalog # BAF1828, filled histogram) or control antibody (BAF108, open histogram), followed by Phycoerythrin-conjugated Streptavidin (F0040). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM2
TREM2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa molecular weight type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. Within the ECD, human TREM2 shares 73% and 74% aa sequence identity with mouse and rat TREM2, respectively. Two closely related transcripts were reported in mouse and designated TREM2a and TREM2b. Soluble forms of the TREM2 ECD are generated by alternative splicing or proteolytic cleavage, and the cytoplasmic domain can be liberated by gamma-Secretase mediated intramembrane cleavage. It is a pattern recognition receptor that binds anionic ligands. A positively charged lysine within the transmembrane segment allows association with the signal adapter protein, DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses, including inhibition of macrophage activation. TREM2 is expressed on macrophages, immature myeloid dendritic cells, osteoclasts, microglia, and adipocytes. It promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. In the CNS, TREM2 binds to ApoE, ApoA1, and ApoB and mediates the clearance of apoptotic neurons, amyloid plaques, and cell debris following demyelination. TREM2 also interacts with and modifies signaling through Plexin A1 on dendritic cells and osteoclasts. Mutations in TREM2 or DAP12 are associated with the development of Alzheimer's disease and Nasu-Hakola disease (NHD/PLOSL) which is characterized by presenile dementia and bone cysts. Soluble TREM2 is elevated in cerebrospinal fluid of patients with active multiple sclerosis (MS), and TREM2 blockade exacerbates disease symptoms in the experimental EAE model of MS.
Product Datasheets
Citations for Human TREM2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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The relationship of soluble TREM2 to other biomarkers of sporadic Alzheimer's disease
Authors: SH Park, EH Lee, HJ Kim, S Jo, S Lee, SW Seo, HH Park, SH Koh, JH Lee
Scientific Reports, 2021;11(1):13050.
Species: Human
Sample Types: Plasma
Applications: Immunoprecipitation -
PLCgamma2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages
Authors: J Obst, HL Hall-Rober, TB Smith, M Kreuzer, L Magno, E Di Daniel, JB Davis, E Mead
Scientific Reports, 2021;11(1):19842.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Detection -
Exercise as a potential modulator of inflammation in patients with Alzheimer's disease measured in cerebrospinal fluid and plasma
Authors: CS Jensen, JM Bahl, LB Østergaard, P Høgh, L Wermuth, A Heslegrave, H Zetterberg, NHH Heegaard, SG Hasselbalc, AH Simonsen
Exp. Gerontol., 2019;121(0):91-98.
Species: Human
Sample Types: Plasma
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Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
Nat Commun, 2019;10(1):1365.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection) -
Plasma levels of soluble TREM2 and neurofilament light chain in TREM2 rare variant carriers
Authors: NJ Ashton, M Suárez-Cal, A Heslegrave, A Hye, C Razquin, P Pastor, R Sanchez-Va, JL Molinuevo, PJ Visser, K Blennow, AK Hodges, H Zetterberg
Alzheimers Res Ther, 2019;11(1):94.
Species: Human
Sample Types: Plasma
Applications: ELISA Capture -
Cerebrospinal fluid soluble TREM2 levels in frontotemporal dementia differ by genetic and pathological subgroup
Authors: IOC Woollacott, JM Nicholas, A Heslegrave, C Heller, MS Foiani, KM Dick, LL Russell, RW Paterson, A Keshavan, NC Fox, JD Warren, JM Schott, H Zetterberg, JD Rohrer
Alzheimers Res Ther, 2018;10(1):79.
Species: Human
Sample Types: CSF
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CSF progranulin increases in the course of Alzheimer's disease and is associated with sTREM2, neurodegeneration and cognitive decline
Authors: M Suárez-Cal, A Capell, MÁ Araque Cab, E Morenas-Ro, K Fellerer, N Franzmeier, G Kleinberge, E Eren, Y Deming, L Piccio, CM Karch, C Cruchaga, K Paumier, RJ Bateman, AM Fagan, JC Morris, J Levin, A Danek, M Jucker, CL Masters, MN Rossor, JM Ringman, LM Shaw, JQ Trojanowsk, M Weiner, M Ewers, C Haass,,
EMBO Mol Med, 2018;0(0):.
Species: Human
Sample Types: CSF
Applications: ELISA Development (Capture) -
A data-driven approach links microglia to pathology and prognosis in amyotrophic lateral sclerosis
Authors: J Cooper-Kno, C Green, G Altschuler, W Wei, JJ Bury, PR Heath, M Wyles, C Gelsthorpe, JR Highley, A Lorente-Po, T Beck, K Doyle, K Otero, B Traynor, J Kirby, PJ Shaw, W Hide
Acta Neuropathol Commun, 2017;5(1):23.
Species: Human
Sample Types: CSF
Applications: ELISA Development (Detection) -
A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells
Authors: MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu
J. Biol. Chem., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Development -
TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer's disease-associated H157Y variant
Authors: P Thornton, J Sevalle, MJ Deery, G Fraser, Y Zhou, S Ståhl, EH Franssen, RB Dodd, S Qamar, B Gomez Pere, LS Nicol, S Eketjäll, J Revell, C Jones, A Billinton, PH St George-, I Chessell, DC Crowther
EMBO Mol Med, 2017;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
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