Human TROP-2 APC-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 77220
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TROP-2
    Accession # P09758
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TROP-2 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 APC-conjugated Monoclonal Antibody
(Catalog # FAB650A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

  • References:
    1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
    2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
    3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
    4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
    5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
  • Long Name:
    Tumor-associated Calcium Signal Transducer 2
  • Entrez Gene IDs:
    4070 (Human); 56753 (Mouse)
  • Alternate Names:
    Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP2GA7331; tumor-associated calcium signal transducer 2
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Estrogen Receptor beta Activation Impairs Prostatic Regeneration by Inducing Apoptosis in Murine and Human Stem/Progenitor Enriched Cell Populations.
    Authors: Hussain S, Lawrence MG, Taylor RA, Lo CY, Bioresource AP, Frydenberg M, Ellem SJ, Furic L, Risbridger GP
    PLoS ONE, 2012;7(7):e40732.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A APC-conjugated Antibody

Ctrl IC003A 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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