Human TROP-2 Antibody

Catalog # Availability Size / Price Qty
MAB650-SP
MAB650-100
MAB650-500
Human TROP-2 Antibody in Data
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Human TROP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TROP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) VCAM‑1 or rhICAM‑1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 77220
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TROP-2
His27-Thr274
Accession # P09758
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TROP‑2 Fc Chimera (Catalog # 650-T2)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of TROP-2 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TROP‑2 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 Monoclonal Antibody (Catalog # MAB650, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

References
  1. Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
  2. Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
  3. Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
  4. Ripani, E. et al. (1998) Int. J. Cancer 76:671.
  5. El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

Product Datasheets

Citations for Human TROP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development
    Authors: Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan
    J Cancer, 2020;11(11):3375-3386.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  2. Development of a sensitive screening method for selecting monoclonal antibodies to be internalized by cells.
    Authors: Yamaguchi M, Nishii Y, Nakamura K, Aoki H, Hirai S, Uchida H, Sakuma Y, Hamada H
    Biochem Biophys Res Commun, 2014;454(4):600-603.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
    Authors: Nakatsukasa M, Kawasaki S, Yamasaki K
    Am. J. Pathol., 2010;177(3):1344-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  4. Epithelial to mesenchymal transition predicts gefitinib resistance in cell lines of head and neck squamous cell carcinoma and non-small cell lung carcinoma.
    Authors: Frederick BA, Helfrich BA, Coldren CD, Zheng D, Chan D, Bunn PA, Raben D
    Mol. Cancer Ther., 2007;6(6):1683-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TROP-2 Antibody

Average Rating: 3 (Based on 2 Reviews)

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Human TROP-2 Antibody
By Anonymous on 12/23/2020
Application: Flow Sample Tested: Breast cancer cell line MCF7 Species: Human

Detection of TROP2 on human breast cancer cell line MCF7. MCF7 cells were treated with 67 nM of the human TROP2 antibody (catalog # MAB650) or mouse IgG2a isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human TROP2 antibody (Blue).


Human TROP-2 Antibody
By Anonymous on 06/08/2020
Application: Binding by Octet Sample Tested: Recombinant protein Species: Human