Human TROP-2 Antibody Summary
Accession # P09758
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TROP‑2 in PC-3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TROP-2 Monoclonal Antibody (Catalog # MAB650, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EpCAM gene. TROP-2 and EpCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. The human TROP-2 protein consists of a putative 26 amino acid (aa) signal sequence, a 248 aa extracellular domain, a 23 aa transmembrane region and a 26 aa cytoplasmic domain. TROP-2 is capable of transducing an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.
- Linnenbach, A.J. et al. (1989) Proc. Natl. Acad. Sci. USA 86:27.
- Linnenbach, A.J. et al. (1993) Mol. Cell. Biol. 13:1507.
- Fornaro, M. et al. (1995) Int. J. Cancer 62:610.
- Ripani, E. et al. (1998) Int. J. Cancer 76:671.
- El Sewedy, T. et al. (1998) Int. J. Cancer 75:324.
Citations for Human TROP-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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miR-488-3p sponged by circ-0000495 and mediated upregulation of TROP2 in head and neck squamous cell carcinoma development
Authors: Y Hao, D Zhang, Y Guo, Z Fu, D Yu, G Guan
J Cancer, 2020;11(11):3375-3386.
Sample Types: Cell Lysate
Applications: Western Blot
Development of a sensitive screening method for selecting monoclonal antibodies to be internalized by cells.
Authors: Yamaguchi M, Nishii Y, Nakamura K, Aoki H, Hirai S, Uchida H, Sakuma Y, Hamada H
Biochem Biophys Res Commun, 2014;454(4):600-603.
Sample Types: Whole Cells
Tumor-associated calcium signal transducer 2 is required for the proper subcellular localization of claudin 1 and 7: implications in the pathogenesis of gelatinous drop-like corneal dystrophy.
Authors: Nakatsukasa M, Kawasaki S, Yamasaki K
Am. J. Pathol., 2010;177(3):1344-55.
Sample Types: Cell Lysates
Epithelial to mesenchymal transition predicts gefitinib resistance in cell lines of head and neck squamous cell carcinoma and non-small cell lung carcinoma.
Authors: Frederick BA, Helfrich BA, Coldren CD, Zheng D, Chan D, Bunn PA, Raben D
Mol. Cancer Ther., 2007;6(6):1683-91.
Sample Types: Whole Cells
Applications: Flow Cytometry
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Detection of TROP2 on human breast cancer cell line MCF7. MCF7 cells were treated with 67 nM of the human TROP2 antibody (catalog # MAB650) or mouse IgG2a isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human TROP2 antibody (Blue).