Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF3586
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Trypsin 1, human Trypsin 2 and human Trypsin 3 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Trypsin 2 (R&D Systems, Catalog # 3586-SE)
Ala16-Ser247
Accession # P07478
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Human Trypsin-1 (Catalog # 3848-SE)

Recombinant Human Trypsin-2 (Catalog # 3586-SE)

Recombinant Human Trypsin-3 (Catalog # 3710-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Trypsin

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes.  Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration.  Trypsin-1 (cationic), -2 (anionic), and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length.  The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule.  Asp194 is linked to enzyme activity, and Tyr154 is sulfated.  Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence.  Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg.  Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity.  Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin.  In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations.  In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio.

Alternate Names
Trypsin

Product Datasheets

Citation for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Newborn screening for cystic fibrosis by use of a multiplex immunoassay.
    Authors: Lindau-Shepard BA, Pass KA,
    Clin. Chem., 2010;56(3):445-50.
    Species: Human
    Sample Types: Whole Blood
    Applications: Immunoassay Development

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Reviews for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

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Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
By Anonymous on 04/17/2018
Application: Luminex XMAP Technology Sample Tested: Adult pancreas Species: Human

used as detector antibody in our assay together with AF3586 combined with Trypsin 1 from another vendor


Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
By Anonymous on 02/22/2016
Application: Luminex xMAP magnetic microspheres technology Sample Tested: Whole blood Species: Human