Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody Summary
Accession # P07478
Recombinant Human Trypsin-1 (Catalog # 3848-SE)
Recombinant Human Trypsin-2 (Catalog # 3586-SE)
Recombinant Human Trypsin-3 (Catalog # 3710-SE)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes. Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration. Trypsin-1 (cationic), -2 (anionic), and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length. The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule. Asp194 is linked to enzyme activity, and Tyr154 is sulfated. Over their mature regions, the three trypsins share 84% aa identity. Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence. Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or
Citation for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Newborn screening for cystic fibrosis by use of a multiplex immunoassay.
Authors: Lindau-Shepard BA, Pass KA,
Clin. Chem., 2010;56(3):445-50.
Sample Types: Whole Blood
Applications: Immunoassay Development
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used as detector antibody in our assay together with AF3586 combined with Trypsin 1 from another vendor