Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunoassay Development

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Trypsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trypsin 2 (R&D Systems, Catalog # 3586-SE)
Ala16-Ser247
Accession # P07478

Specificity

Detects human Trypsin 1, human Trypsin 2 and human Trypsin 3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample:

Recombinant Human Trypsin-1 (Catalog # 3848-SE)

Recombinant Human Trypsin-2 (Catalog # 3586-SE)

Recombinant Human Trypsin-3 (Catalog # 3710-SE)

Reviewed Applications

Read 2 reviews rated 5 using BAF3586 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsin

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes.  Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration.  Trypsin-1 (cationic), -2 (anionic), and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length.  The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule.  Asp194 is linked to enzyme activity, and Tyr154 is sulfated.  Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence.  Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg.  Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity.  Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin.  In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations.  In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio.

Alternate Names

TRY2, TRY8, TRYP2

UniProt

P07478

Additional Trypsin Products

Product Documents for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody

Customer Reviews for Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody (2)

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  • Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
    Name: Anonymous
    Application: Luminex XMAP Technology
    Sample Tested: Adult pancreas
    Species: Human
    Verified Customer | Posted 04/17/2018
    used as detector antibody in our assay together with AF3586 combined with Trypsin 1 from another vendor
    Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody BAF3586
  • Human Trypsin Pan Specific (PRSS1/2/3) Biotinylated Antibody
    Name: Anonymous
    Application: Luminex xMAP magnetic microspheres technology
    Sample Tested: Whole blood
    Species: Human
    Verified Customer | Posted 02/22/2016

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