Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Trypsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Trypsin 2
Ala16-Ser247
Accession # P07478

Specificity

Detects human Trypsin Pan Specific (PRSS1/2/3) in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

Detection of Human Trypsin by Western Blot.

Western blot shows lysates of human pancreas. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Trypsin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human Trypsin by Western Blot.

Western blot shows recombinant human Trypsin 1, 2, and 3. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Trypsin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Trypsin antibody in Human Pancreas by Immunohistochemistry (IHC-P).

Trypsin in Human Pancreas.

Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Trypsin antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P).

Trypsin in Human Pancreatic Cancer Tissue.

Trypsin was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3586) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Trypsin by Immunoprecipitation

Human Trypsin 2/PRSS2 was immunoprecipitated from 500 μg of human pancreas lysates with 12.5 ug Mouse Anti-Human Trypsin 2/PRSS2 Monoclonal Antibody (Catalog # MAB3586). The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody (AF3586) under non-reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas and immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Immunoprecipitation

12.5 µg/500 µg cell lysate
Sample: Human pancreas tissue

Western Blot

1 µg/mL
Sample: Human pancreas and recombinant human Trypsin 1, 2, and 3

Neutralization

Measured by its ability to neutralize Recombinant Human Trypsin 2/PRSS2 (0.02 µg/mL, Catalog # 3586-SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 0.3 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.7 using AF3586 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Trypsin

Trypsin is a general term for any of three 24 kDa gene products that belong to the peptidase S1 family of enzymes. Trypsin in Greek means “rubbing or friction”, and it was chosen here because the first trypsins were extracted from pancreas via a glycerin-based rubbing maceration. Trypsin-1 (cationic), -2 (anionic), and -3 (mesotrypsin) are synthesized as 26 kDa trypsinogens (plus a 35 kDa trypsinogen-3 isoform) that are 247 amino acids (aa) in length. The first 15 aa constitute a signal sequence, followed by an enterokinase-cleavable eight aa propeptide, and a 224 aa mature molecule. Asp194 is linked to enzyme activity, and Tyr154 is sulfated. Over their mature regions, the three trypsins share 84% aa identity.  Mouse trypsin-1 shares 74% aa identity with the human trypsin consensus sequence. Trypsin-1 and -2 cleave peptide bonds carboxylterminal to a Lys or Arg. Trypsin-3 has limited activity on trypsin inhibitors, and thus regulates general trypsin activity. Trypsin-1, -2, and -3 are expressed by multiple tissues, including pancreas (for general digestion), liver, lung and skin. In blood, trypsinogen-1 and -2 circulate at ng/mL concentrations. In pancreatic fluid, trypsin-1, -2, and -3 are found in a 58:40:2 ratio. This antibody did not neutralize the activity of rhTrypsin 1 and rhTrypsin 3 significantly.  

Alternate Names

TRY2, TRY8, TRYP2

UniProt

P07478

Additional Trypsin Products

Product Documents for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Trypsin Pan Specific (PRSS1/2/3) Antibody

Customer Reviews for Human Trypsin Pan Specific (PRSS1/2/3) Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human Trypsin Pan Specific (PRSS1/2/3) Antibody
    Name: Anonymous
    Application: Luminex XMAP technology
    Sample Tested: Adult pancreas
    Species: Human
    Verified Customer | Posted 04/17/2018
    product was used as a primary antibody attached to a solid phase. Works only when combined with Trypsin-1 from another vendor as 50/50 rate
    Human Trypsin Pan Specific (PRSS1/2/3) Antibody AF3586
  • Human Trypsin Pan Specific (PRSS1/2/3) Antibody
    Name: Scott Messenger
    Application: Immunocytochemistry
    Sample Tested: Mouse pancreas in OCT
    Species: Mouse
    Verified Customer | Posted 06/09/2017
    cryosection of mouse pancreas, primary 1/100, secondary 1/200 488. Epi-fluorescence 60 x. signal weak
    Human Trypsin Pan Specific (PRSS1/2/3) Antibody AF3586
  • Human Trypsin Pan Specific (PRSS1/2/3) Antibody
    Name: Scott Messenger
    Application: Western Blot
    Sample Tested: human acini, rat acini and AR42J rat acinar-like cells
    Species: Human and Rat
    Verified Customer | Posted 05/24/2017
    1/1000, 12% gel, 50 ug protein
    Human Trypsin Pan Specific (PRSS1/2/3) Antibody AF3586

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Protocols

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