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Human UbcH6/UBE2E1 Antibody

Catalog #: A-630
3 Citations Datasheet / COA / SDS

Discontinued Product

A-630 has been discontinued.
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Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UbcH6/UBE2E1 Antibody Summary

Species Reactivity
Human
Specificity
Detects UbcH6/UBE2E1 in Western blots.
Source
Polyclonal Rabbit IgG
Purification
Protein A or G purified
Immunogen
Full-length, highly purified human UbcH6 protein
Formulation
Supplied in a solution of PBS and Glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Recommendeddilution range for Western blot analysis is 1:100 to 1:1000.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UbcH6/UBE2E1

UbcH6 mediates the selective degradation of short-lived and abnormal proteins.  The enzyme has high similarity to Ubch5 E2 subfamily, but it does not participate in HECT-domain mediated events.

Entrez Gene IDs
7324 (Human); 22194 (Mouse)
Alternate Names
EC 6.3.2.19; UbcH6; UBE2E1; Ubiquitin carrier protein E1; ubiquitin-conjugating enzyme E2 E1; ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase E1

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Citations for Human UbcH6/UBE2E1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. OTUB1 non-catalytically stabilizes the E2 ubiquitin-conjugating enzyme UBE2E1 by preventing its autoubiquitination
    Authors: N Pasupala, ME Morrow, LT Que, BA Malynn, A Ma, C Wolberger
    J. Biol. Chem., 2018-10-03;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. UbE2E1/UBCH6 Is a Critical in Vivo E2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119
    Authors: K Wheaton, F Sarkari, B Stanly Joh, H Davarineja, O Egorova, L Kaustov, B Raught, V Saridakis, Y Sheng
    J. Biol. Chem, 2017-01-10;292(7):2893-2902.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Ubiquitylation of terminal deoxynucleotidyltransferase inhibits its activity.
    Authors: Maezawa S, Fukushima R, Matsushita T
    PLoS ONE, 2012-07-11;7(7):e39511.
    Species: Human
    Sample Types: Whole Cells

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