Human UBE2K/E2-25K Antibody

Name: Human UBE2K/E2-25K Antibody
Brand: R&D Systems
SKU: A-603

Catalog #: A-603 Datasheet / COA / SDS

Discontinued Product

A-603 has been discontinued.

View all UBE2K/E2-25K products.

All UBE2K/E2-25K Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UBE2K/E2-25K Antibody Summary

Species Reactivity

Human

Specificity

Detects UBE2K/E2‑25K in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Protein A or G purified

Immunogen

Full-length, highly purified human UbcH1 protein

Formulation

Supplied in a solution of PBS and Glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Recommendeddilution range for Western blot analysis is 1:100 to 1:1000.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store unopened vial at -80°C. Avoid repeated freeze-thaw cycles. Aliquot antibody in small volumes prior to freezing.

Background: UBE2K/E2-25K

Biochemically, UBE2K (E2-25K) can catalyze the formation of K48-linked ubiquitin chains in the absence of an E3 ligase. This enzyme has a unique C-terminal UBA domain that plays a critical role in E1 selectivity and chain synthesis. Recently, UBE2K (or E2-25K or HIP2) has been shown to bind to the N- terminus of huntington and may play a role in Huntington’s disease.

Long Name

Ubiquitin-conjugating Enzyme E2

Entrez Gene IDs

3093 (Human); 53323 (Mouse); 289623 (Rat)

Alternate Names

E2(25K); E225K; E2-25K; EC 6.3.2.19; Hip2; HIP-2; huntingtin interacting protein 2; Huntingtin-interacting protein 2; HYPG; LIG; UbcH1; UBE2K; UBE2K-25K; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2 K; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase