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Human UNG Antibody

Catalog #: MAB2287 Datasheet / COA / SDS

Discontinued Product

MAB2287 has been discontinued.
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Detection of Human UNG by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human UNG Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human UNG in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 203627
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human UNG1
Phe85-Leu304
Accession # P13051-2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human UNG antibody by Western Blot. View Larger

Detection of Human UNG by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human UNG Monoclonal Antibody (Catalog # MAB2287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UNG at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: UNG

Human UNG is one of four known human DNA N-glycosylases involved in the removal of uracil from DNA. Human UNG is a monofunctional enzyme that initiates base excision repair (BER) by hydrolyzing the N-glycosidic bond between the uracil base and the sugar-phosphate backbone on the DNA. The resultant abasic or AP site generated by this cleavage is repaired in subsequent steps of the BER process.

Long Name
Uracil N-Glycosylase
Entrez Gene IDs
7374 (Human)
Alternate Names
DGU; DGUEC 3.2.2; HIGM4EC 3.2.2.27; UDG; UDGDKFZp781L1143; UNG; UNG15; UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; UNG2; uracil-DNA glycosylase

Product Datasheets

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Product Datasheet
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