Human UNG Antibody

MAB2287 has been discontinued.
  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human UNG in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 203627
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human UNG1
    Phe85-Leu304
    Accession # P13051-2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human UNG by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human UNG Monoclonal Antibody (Catalog # MAB2287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UNG at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: UNG

Human UNG is one of four known human DNA N-glycosylases involved in the removal of uracil from DNA. Human UNG is a monofunctional enzyme that initiates base excision repair (BER) by hydrolyzing the N-glycosidic bond between the uracil base and the sugar-phosphate backbone on the DNA. The resultant abasic or AP site generated by this cleavage is repaired in subsequent steps of the BER process.

  • Long Name:
    Uracil N-Glycosylase
  • Entrez Gene IDs:
    7374 (Human)
  • Alternate Names:
    DGU; DGUEC 3.2.2; HIGM4EC 3.2.2.27; UDG; UDGDKFZp781L1143; UNG; UNG15; UNG1uracil-DNA glycosylase 1, uracil-DNA glycosylase 2; UNG2; uracil-DNA glycosylase
Related Research Areas

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