Detection of Human UNG by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Human UNG Monoclonal Antibody (Catalog # MAB2287) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for UNG at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human UNG is one of four known human DNA N-glycosylases involved in the removal of uracil from DNA. Human UNG is a monofunctional enzyme that initiates base excision repair (BER) by hydrolyzing the N-glycosidic bond between the uracil base and the sugar-phosphate backbone on the DNA. The resultant abasic or AP site generated by this cleavage is repaired in subsequent steps of the BER process.
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