Human VCAM-1/CD106 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5649A
Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry.
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Product Details
Citations (3)
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Human VCAM-1/CD106 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
Source
Monoclonal Mouse IgG2A Clone # IE10
Purification
Protein A or G purified from ascites
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VCAM-1/CD106 antibody in HuT 78 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of VCAM‑1/CD106 in HuT 78 Human Cell Line by Flow Cytometry. HuT 78 human cutaneous T cell lymphoma cell line was stained with Mouse Anti-Human VCAM-1/CD106 APC-conjugated Monoclonal Antibody (Catalog # FAB5649A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VCAM-1/CD106

Vascular cell adhesion molecule 1 (VCAM-1) is expressed on the surface of activated endothelial cells and macrophages. It binds to leukocyte integrins VLA-4 and alpha 4 beta 7 to promote leukocyte adhesion at sites of inflamed vasculature.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

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Citations for Human VCAM-1/CD106 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. The neovascularization effect of dedifferentiated fat cells
    Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
    Sci Rep, 2020-06-08;10(1):9211.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Measuring Leukocyte Adhesion to (Primary) Endothelial Cells after Photon and Charged Particle Exposure with a Dedicated Laminar Flow Chamber
    Authors: N Erbeldinge, F Rapp, S Ktitareva, P Wendel, AS Bothe, T Dettmering, M Durante, T Friedrich, B Bertulat, S Meyer, MC Cardoso, S Hehlgans, F Rödel, C Fournier
    Front Immunol, 2017-06-01;8(0):627.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016-03-22;291(20):10586-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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