Human VE-Cadherin APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB9381A-100
FAB9381A-025
Human VE-Cadherin APC-conjugated Antibody in Data
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human VE-Cadherin APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
Source
Monoclonal Mouse IgG2B Clone # 123413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of VE-Cadherin antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VE‑Cadherin in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VE-Cadherin APC-conjugated Monoclonal Antibody (Catalog # FAB9381A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VE-Cadherin

Vascular Endothelial (VE)-Cadherin (VE-CAD), also called 7B4 and Cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-Cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-Cadherin proteins share approximately 74% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Product Datasheets

Citation for Human VE-Cadherin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Linking cell function with perfusion: insights from the transcatheter delivery of bone marrow-derived CD133+ cells in ischemic refractory cardiomyopathy trial (RECARDIO)
    Authors: B Bassetti, C Carbucicch, V Catto, E Gambini, E Rurali, A Bestetti, G Gaipa, D Belotti, F Celeste, M Parma, S Righetti, L Biava, M Arosio, A Bonomi, P Agostoni, P Scacciatel, F Achilli, G Pompilio
    Stem Cell Res Ther, 2018;9(1):235.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human VE-Cadherin APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Human VE-Cadherin APC-conjugated Antibody and earn rewards!

Have you used Human VE-Cadherin APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review