Human VE-Cadherin APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 123413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
    Accession # P33151
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of VE‑Cadherin in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VE‑Cadherin APC‑conjugated Monoclonal Antibody (Catalog # FAB9381A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VE-Cadherin

Vascular Endothelial (VE)-Cadherin (VE-CAD), also called 7B4 and Cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-Cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-Cadherin proteins share approximately 74% homology.

  • References:
    1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
    2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    3. Overduin, M. et al. (1995) Science 267:386.
    4. Takeichi, M. (1991) Science 251:1451.
    5. Nose, A. et al. (1987) EMBO J. 6:3655.
    6. Carmeliet, P. et al. (1999) Cell 98:147.
    7. Gory-Faure, S. et al. (1999) Development 126:2093.
  • Long Name:
    Vascular Endothelium Cadherin
  • Entrez Gene IDs:
    1003 (Human); 12562 (Mouse)
  • Alternate Names:
    7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-cadherin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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