Human VE-Cadherin Antibody
Human VE-Cadherin Antibody Summary
no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
Asp48-Gln593
Accession # P33151
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VE-Cadherin
Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
- Carmeliet, P. et al. (1999) Cell 98:147.
- Gory-Faure, S. et al. (1999) Development 126:2093.
Product Datasheets
Citations for Human VE-Cadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
Authors: R Daum, D Visser, C Wild, L Kutuzova, M Schneider, G Lorenz, M Weiss, S Hinderer, UA Stock, M Seifert, K Schenke-La
Cells, 2020;9(3):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
Authors: BL Ecker, A Kaur, SM Douglass, MR Webster, FV Almeida, GE Marino, AJ Sinnamon, MG Neuwirth, GM Alicea, A Ndoye, M Fane, X Xu, MS Sim, GB Deutsch, MB Faries, GC Karakousis, AT Weeraratna
Cancer Discov, 2019;9(1):82-95.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis
Authors: T Neri, E Hiriart, PP van Vliet, E Faure, RA Norris, B Farhat, B Jagla, J Lefrancois, Y Sugi, T Moore-Morr, S Zaffran, RS Faustino, AC Zambon, JP Desvignes, D Salgado, RA Levine, JL de la Pomp, A Terzic, SM Evans, R Markwald, M Pucéat
Nat Commun, 2019;10(1):1929.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling
Authors: Y Kiyan, S Tkachuk, K Kurselis, N Shushakova, K Stahl, D Dawodu, R Kiyan, B Chichkov, H Haller
Sci Rep, 2019;9(1):13591.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages
Authors: J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao
Cell Transplant, 2018;0(0):9636897187803.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function
Authors: K Kulhankova, KJ Kinney, JM Stach, FA Gourronc, IM Grumbach, AJ Klingelhut, W Salgado-Pa
Infect. Immun., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier
Authors: JC Bramley, CG Drummond, NJ Lennemann, CA Good, KS Kim, CB Coyne
mSphere, 2017;2(3):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
Authors: Gabriela Odent Grig
J Tissue Eng Regen Med, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
Cell Stem Cell, 2008;3(1):55-68.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Reviews for Human VE-Cadherin Antibody
Average Rating: 4.5 (Based on 2 Reviews)
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