Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 123413
View all VE-Cadherin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151

Specificity

Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin andno cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human VE‑Cadherin Antibody

Detection of Human VE-Cadherin antibody by Western Blot.

Detection of Human VE‑Cadherin by Western Blot.

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC).

VE‑Cadherin in HUVEC Cells.

VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human VE-Cadherin by Flow Cytometry

Detection of Human VE-Cadherin by Flow Cytometry

Immortalized HAECs retain phenotypic and functional characteristics of primary cells. (A) (Top) Immortalized HAEC confluent monolayers stained with CD31-AF488 antibody (green) and DAPI (blue). (Bottom) Flow cytometry histogram plots of primary or immortalized HAECs labeled with CD31-AF488 antibody. Secondary antibody only (dashed line) and nonstained cells (dotted line) were used as negative controls. (B) (Top) Immortalized HAEC confluent monolayers stained with VE-cadherin-AF488 (green) antibody, Acti-Stain 555 phalloidin (red), and DAPI (blue). (Bottom) Flow cytometry histogram plots of immortalized HAECs labeled with VE-cadherin-AF488 and ZO-1-AF647 antibodies. Secondary antibody only (dashed line) was used as a negative control. (C) Tube forming assay of primary (top) and immortalized (bottom) HAECs at 0 h and 8 h after seeding in Matrigel-coated wells. (D) Acetylated low-density lipoprotein (Ac-LDL) uptake assay of primary HAECs (top), immortalized HAECs (middle), and fibroblasts (negative control; bottom) treated with fluorescently labeled Ac-LDL (red) for 2.5 h and stained with DAPI (blue). (E) IL-8 detection in culture supernatants 24 h after treatment of primary or immortalized HAECs with increasing concentrations of TNF-alpha, IFN-gamma, or IL-6. (F) IL-8 detection in culture supernatants 24 h after treatment of immortalized HAECs with increasing concentrations of IL-2 or IL-1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29229737), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human VE‑Cadherin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells stained in buffer containing Ca2+ and Mg2+

Immunocytochemistry

0.5-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 2 reviews rated 4.5 using MAB9381 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VE-Cadherin

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Gene Symbol

CDH5

UniProt

P33151

Additional VE-Cadherin Products

Product Documents for Human VE‑Cadherin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VE‑Cadherin Antibody

For research use only

Citations for Human VE‑Cadherin Antibody

Customer Reviews for Human VE‑Cadherin Antibody (2)

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  • Human VE-Cadherin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 04/05/2022
    Human VE‑Cadherin Antibody MAB9381
  • Human VE-Cadherin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Human Coronary Artery Endothelial Cells
    Species: Human
    Verified Customer | Posted 09/10/2021
    Human VE‑Cadherin Antibody MAB9381

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Protocols

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FAQs for Human VE‑Cadherin Antibody

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  • Q: Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    A: The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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