Human VE-Cadherin Antibody

Catalog # Availability Size / Price Qty
MAB9381
MAB9381-SP
Human VE-Cadherin Antibody in Data
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Product Details
Citations (10)
FAQs
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Human VE-Cadherin Antibody Summary

Species Reactivity
Human
Specificity
Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and
no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
Source
Monoclonal Mouse IgG2B Clone # 123413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells stained in buffer containing Ca2+ and Mg2+
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VE-Cadherin antibody by Western Blot. View Larger

Detection of Human VE‑Cadherin by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC). View Larger

VE‑Cadherin in HUVEC Cells. VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

References
  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Entrez Gene IDs
1003 (Human); 12562 (Mouse)
Alternate Names
7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin

Product Datasheets

Citations for Human VE-Cadherin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture
    Authors: R Daum, D Visser, C Wild, L Kutuzova, M Schneider, G Lorenz, M Weiss, S Hinderer, UA Stock, M Seifert, K Schenke-La
    Cells, 2020;9(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
    Authors: BL Ecker, A Kaur, SM Douglass, MR Webster, FV Almeida, GE Marino, AJ Sinnamon, MG Neuwirth, GM Alicea, A Ndoye, M Fane, X Xu, MS Sim, GB Deutsch, MB Faries, GC Karakousis, AT Weeraratna
    Cancer Discov, 2019;9(1):82-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis
    Authors: T Neri, E Hiriart, PP van Vliet, E Faure, RA Norris, B Farhat, B Jagla, J Lefrancois, Y Sugi, T Moore-Morr, S Zaffran, RS Faustino, AC Zambon, JP Desvignes, D Salgado, RA Levine, JL de la Pomp, A Terzic, SM Evans, R Markwald, M Pucéat
    Nat Commun, 2019;10(1):1929.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling
    Authors: Y Kiyan, S Tkachuk, K Kurselis, N Shushakova, K Stahl, D Dawodu, R Kiyan, B Chichkov, H Haller
    Sci Rep, 2019;9(1):13591.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages
    Authors: J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao
    Cell Transplant, 2018;0(0):9636897187803.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function
    Authors: K Kulhankova, KJ Kinney, JM Stach, FA Gourronc, IM Grumbach, AJ Klingelhut, W Salgado-Pa
    Infect. Immun., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier
    Authors: JC Bramley, CG Drummond, NJ Lennemann, CA Good, KS Kim, CB Coyne
    mSphere, 2017;2(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
    Authors: Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM
    Cell Stem Cell, 2008;3(1):55-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
    Authors: Rajesh M, Mukhopadhyay P, Batkai S, Hasko G, Liaudet L, Drel VR, Obrosova IG, Pacher P
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H610-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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