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Human VE-Cadherin PE-conjugated Antibody

  (10 citations)     
Datasheet / CoA / SDS
Detection of VE‑Cadherin in HUVEC Human Cells by Flow Cytometry.

Flow Cytometry

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Product Details
Citations (10)
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  • Species Reactivity
    Human
  • Specificity
    Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 123413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
    Asp48-Gln593
    Accession # P33151
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry VE-Cadherin Antibody (123413) [Phycoerythrin]
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Detection of VE‑Cadherin in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VE‑Cadherin PE‑conjugated Monoclonal Antibody (Catalog # FAB9381P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VE-Cadherin

Vascular endothelial (VE)-Cadherin (VE-CAD), also called 7B4 and Cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-Cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-Cadherin proteins share approximately 74% homology.

  • References:
    1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
    2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    3. Overduin, M. et al. (1995) Science 267:386.
    4. Takeichi, M. (1991) Science 251:1451.
    5. Nose, A. et al. (1987) EMBO J. 6:3655.
    6. Carmeliet, P. et al. (1999) Cell 98:147.
    7. Gory-Faure, S. et al. (1999) Development 126:2093.
  • Long Name:
    Vascular Endothelium Cadherin
  • Entrez Gene IDs:
    1003 (Human); 12562 (Mouse)
  • Alternate Names:
    7B4 antigen; 7B4; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); Cadherin-5; CD144 antigen; CD144; CDH5; endothelial-specific cadherin; FLJ17376; Vascular endothelial cadherin; VECadherin; VE-Cadherin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
 Applications
 Sample Type
  1. Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients
    Authors: C Maroun-Eid, A Ortega-Her, J Modrego, M Abad-Cardi, JA García-Don, L Reinares, N Martell-Cl, D Gómez-Garr
    PLoS ONE, 2018;13(1):e0190494.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Somatic GNAQ Mutation is Enriched in Brain Endothelial Cells in�Sturge-Weber Syndrome
    Authors: Lan Huang
    Pediatr. Neurol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
    Authors: Huang L, Nakayama H, Klagsbrun M, Mulliken J, Bischoff J
    Stem Cells, 2015;33(1):133-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells.
    Authors: White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D
    Stem Cells, 2013;31(1):92-103.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium.
    Authors: Pelosi E, Castelli G, Martin-Padura I, Bordoni V, Santoro S, Conigliaro A, Cerio A, De Santis Puzzonia M, Marighetti P, Biffoni M, Alonzi T, Amicone L, Alcalay M, Bertolini F, Testa U, Tripodi M
    PLoS ONE, 2012;7(12):e51109.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression.
    Authors: Ahrens I, Domeij H, Topcic D
    PLoS ONE, 2011;6(8):e23210.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.
    Authors: Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
    Nature, 2010;468(7325):824-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A.
    Authors: Narazaki M, Segarra M, Tosato G
    Blood, 2008;111(8):4126-36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages.
    Authors: Porat Y, Porozov S, Belkin D, Shimoni D, Fisher Y, Belleli A, Czeiger D, Silverman WF, Belkin M, Battler A, Fulga V, Savion N
    Br. J. Haematol., 2006;135(5):703-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Structural and functional studies of the endothelial activation antigen endothelial leucocyte adhesion molecule-1 using a panel of monoclonal antibodies.
    Authors: Pigott R, Needham LA, Edwards RM
    J. Immunol., 1991;147(1):130-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Mouse IgG2B PE-conjugated Antibody

 Ctrl IC0041P 24  
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Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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