Human VIP R2 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5416A-025
Detection  of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and  eGFP by Flow Cytometry.
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Human VIP R2 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human VIP R2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 476031
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human VIP R2
Glu24-Ile438
Accession # P41587
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and  eGFP by Flow Cytometry. View Larger

Detection of VIP R2 in HEK293 Human Cell Line Transfected with Human VIP R2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human VIP R2 and eGFP were stained with either (A) Mouse Anti-Human VIP R2 APC‑conjugated Monoclonal Antibody (Catalog # FAB5416A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: VIP R2

Human VIP R2 (Vasoactive Intestinal Polypeptide Receptor 2), also known as VPAC2 is a 60-70 kDa seven transmembrane glycoprotein receptor that belongs to the G protein coupled receptor 2 family of proteins. The mature chain contains in its first extracellular region a hormone receptor domain (aa 49‑114) which includes three N-linked glycosylation sites and a "cilia targeting signal" comprised of and Arg-Asp-Tyr-Arg sequence. Human VIP R2 shares 85% aa sequence identity with mouse and rat VIP R2. In the extracellular domains, human VIP R2 shares 88% and 31% aa sequence identity with mouse VIP R2 and human VIP R1, respectively. VIP R2 is expressed predominantly in skeletal muscle, CD4+ T cells, smooth muscle cells, plasmacytoid dendritic cells, hepatic progenitor cells, monocytes, synoviocytes and select neurons. VIP R1 and VIP R2 bind VIP with equal affinity, but the two receptors are not redundant. VIP R1 interacts with RAMPs; VIP R2 does not. In the dentate gyrus, VIP R1 drives stem cells into a granule cell phenotype while VIP R2 maintains a Nestin+ phenotype.

Long Name
Vasoactive Intestinal Peptide Receptor 2
Entrez Gene IDs
7434 (Human); 22355 (Mouse); 29555 (Rat)
Alternate Names
FLJ16511; Helodermin-preferring VIP receptor; PACAP type III receptor; PACAPR3; PACAP-R3; PACAP-R-3; Pituitary adenylate cyclase-activating polypeptide type III receptor; vasoactive intestinal peptide receptor 2; vasoactive intestinal polypeptide receptor 2; VIP R2; VIP2R; VIPR2; VIP-R-2; VPAC2; VPCAP2R

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