Human WISP-1/CCN4 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human WISP‑1/CCN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse WISP-1 is observed and less than 1% cross-reactivity with recombiant human (rh) CTGF and rhNOV is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human WISP‑1/CCN4
    Thr23-Asn367
    Accession # O95388
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human WISP‑1/CCN4 (Catalog # 1627-WS)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
WISP‑1/CCN4 in Human Breast Cancer Tissue. WISP‑1/CCN4 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human WISP‑1/CCN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1627) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WISP-1/CCN4
Human WISP-1 (Wnt-induced secreted protein-1; also CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1‑5). It is synthesized as a 367 aa precursor that contains a series of structural homology modules. Following a 22 amino acid (aa) signal sequence, there is a 68 aa IGFBP-like domain (aa 53‑120), a 57 aa von Willebrand factor type C (VWC) module (aa 126‑182), a 40 aa TSP type I domain (aa 220‑259) and a 75 aa, C-terminal cysteine knot motif (aa 273‑347). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). At least five alternative splice forms are known for WISP-1. One is 30 kDa in size, 258 aa in length, and shows a substitution of a His for aa 95‑182. This removes the VWC domain (2, 6). A second isoform is 155 aa in length and shows a frameshift at Arg 117 with a unique 38 aa C‑terminal extension. A third is 195 aa in length and shows a 31 aa substitution for the first 203 aa of the full length precursor (6). This retains the VWC and cysteine knot domains. A fourth shows a 43 aa substitution for aa 117‑367 for a total length of 163 aa. This effectively removes everything but the IGFBP-like domain (7). The last splice form contains a deletion of aa 25‑269 for a total length of 122 aa. Thus, only the signal sequence and cysteine knot motifs are retained (8). This leaves only the IGFBP-like domain (9). Full-length mature human WISP-1 is 85% aa identical to both mouse and rat WISP-1. WISP-1 is expressed by osteoblasts and may contribute to fracture healing by promoting bone cell formation (10, 11).
  • References:
    1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
    2. Tanada, S. et al. (2001) Oncogene 20:5525.
    3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
    4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
    5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
    6. Li, Z. (2005) GenBank Accession # AAP43925.
    7. Li, Z. (2005) GenBank Accession # AAP43926.
    8. Li, Z. (2005) GenBank Accession # AAP43924.
    9. Cervello, M. et al. (2004) Ann. N.Y. Acad. Sci. 1028:432.
    10. French, D.M. et al. (2004), Am. J. Pathol. 165:855.
    11. Parisi, M.S. et al. (2006) Bone 38:671.
  • Long Name:
    Wnt-1 Induced Secreted Protein 1
  • Entrez Gene IDs:
    8840 (Human); 22402 (Mouse)
  • Alternate Names:
    CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Macrophage-derived IL-10 mediates mucosal repair by epithelial WISP-1 signaling
    Authors: M Quiros, H Nishio, PA Neumann, D Siuda, JC Brazil, V Azcutia, R Hilgarth, MN O'Leary, V Garcia-Her, G Leoni, M Feng, G Bernal, H Williams, PH Dedhia, C Gerner-Smi, J Spence, CA Parkos, TL Denning, A Nusrat
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
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