Human Wnt-10b Antibody

Recombinant Monoclonal Antibody
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Product Details
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Human Wnt-10b Antibody Summary

Species Reactivity
Detects human Wnt-10b in direct ELISAs
Recombinant Monoclonal Rat IgG Clone # 904902R
Protein A or G purified from cell culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Wnt-10b
Accession # O00744
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human Wnt‑10b Monoclonal Antibody (Catalog # MAB71963).

This product is intended for assay development on various assay platforms requiring antibody pairs.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10b

Wnt-10b (also known as Wnt-12) is a 42‑44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97‑99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5‑7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5‑7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

  1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
  2. Katoh, M. (2008) Curr. Drug Targets 9:565.
  3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
  4. Swiss Prot Accession # O00744.
  5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
  6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
  7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
  8. Wright, W.S. et al. (2007) Diabetes 56:295.
  9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
  10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
  11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
  12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
  13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
  14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.
Long Name
Wingless-type MMTV Integration Site Family, Member 10B
Entrez Gene IDs
7480 (Human); 22410 (Mouse)
Alternate Names
protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12

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