Human Wnt‑16b Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # FAB7790AFP555

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 948309
View all Wnt-16b Primary Antibodies »

Product Specifications

Specificity

Detects human Wnt-16b in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Wnt‑16b Alexa Fluor™ Plus 555‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-16b

Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).

References

  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  9. Binet, R. et al. (2009) Cancer Res. 69:9183.
  10. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  11. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  12. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  13. Mazieres, J. et al. (2005) Oncogene 24:5396.

Long Name

Wingless-type MMTV Integration Site Family, Member 16B

Alternate Names

WNT16, Wnt16b

Entrez Gene IDs

51384 (Human); 93735 (Mouse); 500047 (Rat)

Gene Symbol

WNT16

UniProt

Q9UBV4 (Human)

Additional Wnt-16b Products

Product Documents for Human Wnt‑16b Alexa Fluor™ Plus 555‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Wnt‑16b Alexa Fluor™ Plus 555‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Wnt Ligands (Wnts)

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