Human Wnt-16b Antibody (948309)

R&D Systems | Catalog # MAB7790

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 948309
View all Wnt-16b Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Wnt-16
Asn30-Lys365
Accession # Q9UBV4

Specificity

Detects human Wnt-16b in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Wnt-16b Antibody (948309)

Wnt-16b antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

Wnt‑16b in HEK293 Human Cell Line.

Wnt-16b was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Wnt-16b Monoclonal Antibody (Catalog # MAB7790) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Wnt-16b Antibody (948309)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB7790 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-16b

Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).

References

  1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
  2. Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
  3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
  4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
  5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
  6. Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
  7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
  8. Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
  9. Binet, R. et al. (2009) Cancer Res. 69:9183.
  10. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
  11. Sun, Y. et al. (2012) Nat. Med. 18:1359.
  12. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
  13. Mazieres, J. et al. (2005) Oncogene 24:5396.

Long Name

Wingless-type MMTV Integration Site Family, Member 16B

Alternate Names

WNT16, Wnt16b

Entrez Gene IDs

51384 (Human); 93735 (Mouse); 500047 (Rat)

Gene Symbol

WNT16

UniProt

Q9UBV4 (Human)

Additional Wnt-16b Products

Product Documents for Human Wnt-16b Antibody (948309)

Certificate of Analysis

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Product Specific Notices for Human Wnt-16b Antibody (948309)

For research use only

Related Research Areas

Wnt Ligands (Wnts)

Customer Reviews for Human Wnt-16b Antibody (948309) (2)

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  • Human Wnt-16b Antibody
    Name: Anonymous
    Application: cellular movement
    Sample Tested: human
    Species: Human
    Verified Customer | Posted 05/20/2023
    Human Wnt-16b Antibody (948309) MAB7790
  • Human Wnt-16b Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: wnt16b-overexpressed HEK29 cell
    Species: Human
    Verified Customer | Posted 08/25/2021
    Human Wnt-16b Antibody (948309) MAB7790

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