Human Wnt-16b Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Wnt-16b in ELISA.
  • Source
    Monoclonal Mouse IgG2B Clone # 948309
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Wnt-16
    Asn30-Lys365
    Accession # Q9UBV4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Wnt‑16b in HEK293 Human Cell Line. Wnt‑16b was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human Wnt‑16b Monoclonal Antibody (Catalog # MAB7790) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-16b
Wnt‑16 is a 40 kDa protein within the Wnt family of secreted, highly conserved, cysteine‑rich, palmitoylated cell signaling glycoproteins that play important roles in vertebrate developmental pattern formation, cell fate decision, axon guidance, and tumor formation (1‑3). Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein (2, 3). Wnt‑16b is the more conserved isoform and is widely expressed, while Wnt‑16a is expressed mainly in the human pancreas (3). Mature human Wnt‑16b shares 92%, 93%, and 95% aa sequence identity with mouse/rat, rabbit/porcine/equine, and bovine Wnt‑16, respectively. Wnt‑16 expression is detected on uterine stroma adjacent to the luminal epithelium during implantation (4). It is up‑regulated during the first embryonic lymphoid progenitor differentiation (5). Congenital heart defects correlate with elevated Wnt‑16 in mouse embryos and human amniotic fluid (6). Low cortical bone thickness and bone mineral density correlate with deletion of Wnt‑16 in mice and a Wnt‑16 missense SNP in humans (7). Wnt‑16 is over‑expressed in cells undergoing replicative senescence, and is up‑regulated in articular cartilage by injury and osteoarthritis (8, 9). Wnt‑16b expression in skin is up‑regulated in human basal cell carcinomas, enhancing cell survival (10). Its expression is also up‑regulated by DNA damage (radiation and chemotherapy) in stroma surrounding prostate tumors, causing enhanced survival and treatment resistance in the tumor cells (11). Pre‑B acute lymphoblastic leukemia with t(1;19) translocation, creating an E2A‑Pbx1 fusion protein, also causes up‑regulation of Wnt‑16 that confers resistance to apoptosis (12, 13). Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported (8‑10).
  • References:
    1. Clevers, H. and R. Nusse (2012) Cell 149:1192.
    2. Katoh, H. and M. Katoh 2005) Oncol. Rep. 13:771.
    3. Fear, M.W. et al. (2000) Biochem. Biophys. Res. Commun. 278:814.
    4. Hayashi, K. et al. (2009) Biol. Reprod. 80:989.
    5. Corrigan, P.M. et al. (2009) Stem Cells Dev. 18:759.
    6. Nath, A.K. et al. (2009) PLoS ONE 4:e4221.
    7. Zheng, H.F. et al. (2012) PLoS Genet. 8:31002745.
    8. Dell’accio, F. et al. (2008) Arthritis Rheum. 58:1410.
    9. Binet, R. et al. (2009) Cancer Res. 69:9183.
    10. Teh, M.T. et al. (2006) J. Cell Sci. 120:330.
    11. Sun, Y. et al. (2012) Nat. Med. 18:1359.
    12. McWhirter, J.R. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11464.
    13. Mazieres, J. et al. (2005) Oncogene 24:5396.
  • Long Name:
    Wingless-type MMTV Integration Site Family, Member 16B
  • Entrez Gene IDs:
    51384 (Human); 93735 (Mouse); 500047 (Rat)
  • Alternate Names:
    WNT16; Wnt16b; Wnt-16b
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