Intracellular Staining by Flow Cytometry
|Detection of Wnt‑9a in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human Wnt‑9a Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3157G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram).To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
Wnt-9a (Wingless integration site family member 9a), formerly Wnt-14, is a 45-47 kDa member of the Wnt family of proteins, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in both embryo and adult. It is found in embryonic mesenchyme adjacent to cartilage, and in lung at the tips of developing bronchioles. In adult, it is found throughout the liver, in hepatocytes, stellate sinusoidal cells, biliary epithelium and endothelium. It also appears in colonic epithelium and in many tumor cell types. It would appear that Wnt-9a binds to Fzd-7 and -9. Human Wnt-9a shares 98% aa sequence identity with mouse and rat Wnt-9a.
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