Huntingtin Antibody (7B9C8)

Novus Biologicals | Catalog # NBP3-15642

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B9C8 expressed in HEK293
View all Huntingtin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Huntingtin (P42858). MATLEKLMKAFESLKSFQQQQQQQQQQQQQQQQQQQQQPPPPPPPPPPPQLPQPPPQAQPLLPQPQPPPPPPPPPPGPAVAEEPLHRPKKELSATKKDRV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Huntingtin Antibody (7B9C8)

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642]

Western Blot: Huntingtin Antibody (7B9C8) [NBP3-15642] - Western blot analysis of extracts of various cell lines, using Huntingtin antibody (NBP3-15642) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642]

Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642]

Immunohistochemistry-Paraffin: Huntingtin Antibody (7B9C8) [NBP3-15642] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Huntingtin Rabbit mAb (NBP3-15642) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Huntingtin Antibody (7B9C8)

Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] -

Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
Huntingtin Antibody (7B9C8)

Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] -

Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Huntingtin Antibody (7B9C8)

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] -

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Huntingtin Antibody (7B9C8)

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] -

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Huntingtin Antibody (7B9C8)

Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] -

Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunofluorescence analysis of Rat brain tissue using Huntingtin Rabbit mAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.
Huntingtin Antibody (7B9C8)

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] -

Immunohistochemistry: Huntingtin Antibody (7B9C8) [Huntingtin] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Huntingtin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Huntingtin Antibody (7B9C8)

Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] -

Western Blot: Huntingtin Antibody (7B9C8) [Huntingtin] - Western blot analysis of various lysates using Huntingtin Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Applications for Huntingtin Antibody (7B9C8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Huntingtin

Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15) gene. Huntington's disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4).

Long Name

Huntingtin

Alternate Names

HD, HTT, IT15

Gene Symbol

HTT

Additional Huntingtin Products

Product Documents for Huntingtin Antibody (7B9C8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Huntingtin Antibody (7B9C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Huntingtin Antibody (7B9C8)

There are currently no reviews for this product. Be the first to review Huntingtin Antibody (7B9C8) and earn rewards!

Have you used Huntingtin Antibody (7B9C8)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies